Method of using autologous fibroblasts to promote healing of wounds and fistulas
The present invention provides a method of using autologous fibroblasts to promote wound or fistula healing in an animal.
1. A method of promoting healing of a fistula in an animal, wherein said fistula has a fistula tract opening and is susceptible to healing upon administration of autologous fibroblasts, which method comprises:
(a) obtaining autologous fibroblasts, and
(b) administering the autologous fibroblasts in the form of an injection into the fistula tract opening, wherein the said autologous fibroblasts promote healing of the fistula.
2. The method of claim 1 , wherein said animal is a human.
3. The method of claim 1 , wherein said autologous fibroblasts are derived from a tissue which is the same type of tissue as a tissue in which the fistula exists.
4. The method of claim 1 , wherein said fibroblasts are cultured in the animal's own serum.
5. The method of claim 1 , wherein said fibroblasts are passaged in culture less than about ten times.
6. The method of claim 5 , wherein said fibroblasts are passaged in culture from about four to about six times.
7. The method of claim 1 , wherein about 20 million autologous fibroblasts are administered per administration.
8. The method of claim 1 , wherein said fistula is an iatrogenic fistula.
9. The method of claim 1 , wherein said fistula is a spontaneous fistula.
10. The method of claim 9 , wherein said fistula is due to infection.
11. The method of claim 9 , wherein the said fistula is due to inflammation.
12. The method of claim 9 , wherein said fistula is due to ischaemia.
13. The method of claim 9 , wherein said fistula is due to carcinoma.
14. The method of claim 9 , wherein said fistula is due to radiation.
15. The method of claim 9 , wherein said fistula is an enterocutaneous fistula.
16. The method of claim 15 , wherein said fistula is a gastric, duodenal, pancreatic, jejunal, ileal, or colonic fistula.
17. The method of claim 1 , wherein said fistula is a tracheo-esophageal fistula.
18. The method of claim 1 , wherein said fistula is a tracheocutaneous fistula.
19. The method of claim 1 , wherein said fistula is an esophagocutaneous fistula.
20. The method of claim 1 , wherein said fistula is a bronchopleural fistula.
21. The method of claim 1 , wherein said fistula is an anal fistula.
22. The method of claim 1 , wherein said autologous fibroblasts are administered by injection of the external fistula tract opening and internal endoscopic injection.