Methods for improving seeds
View Patent ↗The invention provides methods of modulating seed mass and other traits in plants. The methods involve producing transgenic plants comprising a recombinant expression cassette containing an ADC nucleic acid linked to a plant promoter.
1. A method of increasing fatty acid content in a seed of a plant, the method comprising:
providing a first plant comprising a recombinant expression cassette containing a plant promoter operably linked to a nucleic acid sequence selected from a group consisting of SEQ ID NO:3, SEQ ID NO:100, and SEQ ID NO:107, and which nucleic acid encodes a polypeptide that modulates fatty acid content;
selfing the first plant or crossing the first plant with a second plant, thereby producing a plurality of seeds; and
selecting seed with increased fatty acid content.
2. The method of claim 1 , wherein the first and second plants are the same species.
3. The method of claim 1 , wherein the first and second plants are members of the family Brassicaceae.
4. The method of claim 1 , wherein the first and second plants are members of the family Solanaceae.
5. The method of claim 1 , wherein the plant promoter is a constitutive promoter.
6. The method of claim 5 , wherein the promoter is a CaMV 35S promoter.
7. The method of claim 1 , wherein the promoter is a tissue-specific promoter.
8. The method of claim 7 , wherein the promoter is ovule-specific.
9. A seed produced by the method of claim 1 , wherein the seed contains the recombinant expression cassette.