Imaging methods for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices
Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.
1. A method of detecting a light beam comprising:
a step of spatially separating via a means for spatially separating a small portion of the light beam from a an adjacent remainder of the light beam to provide a spatially separated light beam and a remainder light beam;
a step of transmitting the spatially separated light beam to a means for spectroscopy optically connected to the means for separating the light beam; and,
a step of transmitting the remainder light beam to a means for imaging optically connected to receive the remainder light beam and to provide an image therefrom.
2. The method of claim 1 wherein the method further comprises a step of displaying on a means for displaying a spectrum from the means for spectroscopy and the image from the means for imaging.
3. The method of claim 2 wherein the means for spectroscopy is located outside of the light beam and the means for separating the light beam comprises a means for light redirection sized and located to intercept a small area of the light beam and change the direction of such small area toward the means for spectroscopy, and wherein the method further comprises imparting via the means for light redirection a small residual image in the remainder light beam corresponding to the location of the means for light redirection in the light beam.
4. The method of claim 3 wherein the method further comprises a step of contemporaneously displaying both the image from the means for imaging and the spectrum from the means for spectroscopy on a single means for displaying.
5. The method of claim 4 wherein the method is implemented via a means for controlling operably connected to the means for imaging and the means for spectroscopy and containing computer-implemented programming that controls the means for imaging and the means for spectroscopy.