Method for direct detection of fungal pathogens
View Patent ↗The present invention relates to a method and diagnostic kit for directly detecting the presence of a specific species of mold/fungi in a sample, particularly a species which is toxic to humans or animals including pets and livestock. The method involves releasing any fungal DNA from the sample and subjecting it to PCR amplification without the need for purifying the DNA.
1. A method for detecting the presence of Stachybotyrs chartarum in a sample, comprising:
releasing DNA from any fungi present in the sample;
subjecting the released DNA directly to amplification by polymerase chain reaction (PCR) without purifying the DNA, wherein the PCR amplification of any released DNA is performed using at least one pair of primers specific for Stachybotyrs chartarum ; and
detecting the presence of Stachybotyrs chartarum by visualizing any PCR amplification product to determine whether or not the PCR amplification product corresponds to an expected product from amplification of the DNA of Stachybotyrs chartarum with said at least one pair of primers, wherein said at least one pair of primers comprises a pair of primers which comprises the nucleotide sequences of SEQ ID NOs:3 and 4.
2. The method of claim 1 , wherein the sample is obtained from construction or building material.
3. The method of claim 1 , wherein the sample is an air sample.