Immunoassay with extended dynamic range
The present invention provides a method of performing a competitive assay for the detection and quantification of an analyte over an extended dynamic range. This is achieved by a multi-step sample addition method whereby different concentrations of sample are added at different times during the assay that produces a dose-response curve with multiple windows of detection. This multi-step sample addition method causes the dose-response curve of the composite assay to broaden, dramatically increasing the assay dynamic range.
1. A method of performing a competitive assay for a target analyte in a sample which may contain a concentration of the target analyte, comprising the steps of:
a) combining in a vessel a first volume containing a first quantity of said sample and a first quantity of dilution buffer, a first reagent volume containing a known concentration of the target analyte conjugated to a an enzyme, and a second reagent volume containing one or more substrates upon which said enzyme acts and receptors having affinity for the target analyte, wherein said target analyte conjugated to said enzyme binds to said receptors;
b) thermally incubating said vessel for a first time interval;
c) adding a second volume containing a second quantity of said sample and a second quantity of dilution buffer to said vessel, whereby a ratio of an amount of said sample in said second volume to the amount of said sample in said first volume is equal to a prescribed dilution factor;
d) thermally incubating said vessel for a second time interval, during which time an assay signal generated by the action of said enzyme on said one or more substrates is measured and a rate of change of said assay signal is determined, wherein the binding of said target analyte conjugated to said enzyme with said receptors decreases said assay signal, and wherein said signal is measured prior to achieving chemical equilibrium; and
e) relating said rate of change of said assay signal to a concentration of said target analyte in said sample using a pre-determined dose-response curve.
2. The method according to claim 1 wherein said second quantity of dilution buffer is zero.
3. The method according to claim 1 wherein said first and second quantities of dilution buffer are zero and a difference between said first and second quantities of sample is employed to obtain said prescribed dilution factor.
4. The method according to claim 1 wherein the assay is a homogeneous assay.
5. The method according to claim 1 wherein said first volume and said first reagent volume are added to said vessel prior to the addition of said second reagent volume.
6. The method according to claim 1 wherein said first volume and said second reagent volume are added to said vessel prior to the addition of said first reagent volume.
7. The method according to claim 1 wherein said first reagent volume and said second reagent volume are added to said vessel prior to the addition of said first volume.
8. The method according to claim 1 wherein said vessel is vortexted prior to said first thermal incubation time interval.
9. The method according to claim 1 wherein said vessel is vortexted prior to said second thermal incubation time interval.
10. The method according to claim 1 wherein the prescribed dilution factor is chosen to obtain a dose-response curve with a linear response over a wide range of target analyte concentrations.
11. The method according to claim 1 wherein the prescribed dilution factor is chosen to obtain a dose-response curve with two separated linear responses over two different ranges of target analyte concentrations.
12. The method according to claim 1 including measuring an initial assay signal during or after said first time interval and before step c), and relating said initial assay signal to a concentration of said target analyte in said sample using a pre-determined dose-response curve for said initial assay signal.
13. A method of performing a competitive assay for a target analyte in a sample which may contain a concentration of the target analyte, comprising the steps of:
a) performing a first sample addition step including combining in a vessel a first volume containing a first quantity of said sample and a first quantity of dilution buffer, and a first reagent containing a known concentration of the target analyte conjugated to an enzyme, and a second reagent volume containing one or more substrates upon which said enzyme acts and receptors having affinity for the target analyte, wherein said target analyte conjugated to said enzyme binds to said receptors;
b) thermally incubating said vessel for a first time interval;
c) performing one or more additional sample addition and incubation steps including adding an additional volume containing an additional quantity of said sample and an additional quantity of dilution buffer to said vessel, whereby the ratio of an amount of said sample in said additional volume to the amount of said sample used in an immediately preceding sample addition step is equal to a prescribed dilution factor and thermally incubating said vessel for an additional time interval;
d) performing a final sample addition step including adding a final volume containing a final quantity of said sample and a final quantity of dilution buffer to said vessel, whereby the ratio of an amount of said sample in said final volume to the amount of said sample used in an immediately preceding sample addition step is equal to a prescribed dilution factor;
e) thermally incubating said vessel for a final time interval, during which time an assay signal generated by the action of said enzyme on said one more substrates is measured and a rate of change of said assay signal is determined, wherein the binding of said target_analyte conjugated to said enzyme with said receptors decreases said assay signal, and wherein said signal is measured prior to achieving chemical equilibrium; and
f) relating said rate of chance of said assay signal to a concentration of said target analyte in said sample using a pre-determined dose-response curve.
14. The method according to claim 13 wherein the assay is a homogeneous assay.
15. The method according to claim 13 wherein the first volume and said first reagent volume are added to said vessel prior to the addition of said second reagent volume.
16. The method according to claim 13 wherein the first volume and said second reagent volume are added to said vessel prior to the addition of said first reagent volume.
17. The method according to claim 13 wherein said first reagent volume and said second reagent volume are added to said vessel prior to the addition of said first volume.
18. The method according to claim 13 wherein said vessel is vortexted prior to said first thermal incubation time interval.
19. The method according to claim 13 wherein said vessel is vortexted prior to said additional thermal incubation time interval and said final incubation time interval.
20. The method according to claim 13 wherein said vessel is vortexted prior to each of said additional thermal incubation time intervals and said final incubation time interval.
21. The method according to claim 13 wherein the addition of said sample to said dilution buffer is employed to dilute the sample and obtain said prescribed dilution factors.
22. The method according to claim 13 wherein a difference in volume between said first, additional and final volumes is employed to obtain said prescribed dilution factors.
23. The method according to claim 13 wherein the prescribed dilution factors are chosen to obtain a dose-response curve with a linear response over a wide range of target analyte concentrations.
24. The method according to claim 13 wherein the prescribed dilution factors are chosen to obtain a dose-response curve with three separated linear responses over three different ranges of target analyte concentrations.
25. The method according to claim 13 wherein the prescribed dilution factors are chosen to obtain a dose-response curve with multiple separated linear responses over multiple different ranges of target analyte concentrations.
26. The method according to claim 13 including measuring an initial assay signal during or after said first time interval and before step c), and relating said initial assay signal to a concentration of said target analyte in said sample using a pre-determined dose-response curve for said initial assay signal.
27. A method of performing a competitive assay for a target analyte in a sample which may contain a concentration of the target analyte, comprising the steps of:
a) combining in a vessel a first volume containing a first quantity of said sample and a first quantity of dilution buffer, and a first reagent volume containing a known concentration of the target analyte conjugated to an enzyme, and a second reagent volume containing one or more substrates upon which said enzyme acts and receptors having affinity for the target analyte, wherein said target analyte conjugated to said enzyme binds to said receptors;
b) thermally incubating said vessel for a first time interval, during which time a first assay signal generated by the action of said enzyme on said one more substrates is measured;
c) estimating said concentration of the target analyte in said sample by relating said measured first assay signal to said concentration of said target analyte in said sample using a pre-determined dose-response curve corresponding to said first assay signal;
d) calculating an optimal dilution factor by dividing a concentration at which said pre-determined dose-response curve has a maximal slope by said estimated concentration of said target analyte in said sample;
e) adding a second volume containing a second quantity of said sample and a second quantity of dilution buffer to said vessel, whereby a ratio of an amount of said sample in said second volume to the amount of said sample in said first volume is equal to said optimal dilution factor;
f) thermally incubating said vessel for a second time interval, during which time a second assay signal generated by the presence of said enzyme conjugated to said target analyte is measured and a rate of change of said second assay signal is determined, wherein the binding of said target analyte conjugated to said enzyme with said receptors decreases said assay signal, and wherein said signal is measured prior to achieving chemical equilibrium; and
g) relating said rate of change of said second assay signal to said concentration of said target analyte in said sample using a dose-response curve corresponding to said second assay signal.
28. The method according to claim 27 wherein in the event that said estimated concentration of the target analyte obtained in step c) is either zero or indiscernible from zero as a result of a signal-to-noise ratio of said measurement, said optimal dilution factor is chosen to be equal to a fixed value.
29. The method according to claim 27 wherein the assay is a homogeneous assay.
30. The method according to claim 27 wherein said first volume and said first reagent volume are added to said vessel prior to the addition of said second reagent volume.
31. The method according to claim 27 wherein said first volume and said second reagent volume are added to said vessel prior to the addition of said first reagent volume.
32. The method according to claim 27 wherein said first reagent volume and said second reagent volume are added to said vessel prior to the addition of said first volume.
33. The method according to claim 27 wherein said vessel is vortexted prior to said first thermal incubation time interval.
34. The method according to claim 27 wherein said vessel is vortexted prior to said second thermal incubation time interval.
35. The method according to claim 27 wherein the addition of the sample to the dilution buffer is employed to dilute the sample and obtain said optimal dilution factor.
36. The method according to claim 27 wherein a difference in volume between said first and second volumes is employed to obtain said optimal dilution factor.
37. The method according to claim 27 wherein a pre-defined dose-response curve is obtained at a fixed dilution factor chosen to give a dynamic range that spans a specific range of target analyte concentrations where concentrations of the target analyte in unknown samples are expected to lie, whereby signals obtained from a set of known dose-responses are plotted against their concentration, and fitting said signals and concentrations to the mathematical function given by
y
=
a
0
+
a
1
(
1
-
(
a
2
x
)
a
3
)
+
a
4
(
1
-
(
a
5
x
)
a
6
)
where y is the assay signal, x is the analyte concentration, and a 0 a 6 are unknown fitting parameters, wherein said unknown fitting parameters are obtained by mathematically fitting said signals and concentrations to said mathematical function, wherein said dose-response curve is obtained from said pre-determined dose-response curve by replacing said parameter a 5 by an expression a 2 /f, where f is equal to said optimal dilution factor.