Systems and methods for transgenic and targeted mutagenesis screening
The present invention provides a method and apparatus for to conduct transgenic and targeted mutagenesis screening of genomic DNA. This invention also provides a system for screening DNA for a designated genetic sequence. The system includes a computer having a processor, memory and web browser, wherein the computer receives instructions concerning the designated genetic sequence and other screening parameter selected from a remote user via a form of electronic communication, and an automatic screening device that analyzes samples of genomic DNA for the designated sequence.
1. A method to obtain purified mammalian genomic DNA from a mammalian tissue sample using magnetic particles comprising:
(a) treating said mammalian tissue sample to obtain a lysate containing cellular debris including mammalian genomic DNA wherein the step of treating said tissue sample involves treating said tissue sample with a lysis buffer wherein said lysis buffer includes proteinase K under conditions to obtain a lysate;
(b) binding said mammalian genomic DNA to said magnetic particles in the presence of said lysate; and
(c) separating said mammalian genomic DNA from cellular debris using magnetic particles.
2. The method of claim 1 wherein the step of binding said mammalian genomic DNA involves treating said lysate with a binding buffer under conditions to bind said mammalian genomic DNA to said magnetic particles.
3. The method of claim 1 further comprising the step of:
(d) disassociating said mammalian genomic DNA from said magnetic particles.