Bovine immunodeficiency virus (BIV) based vectors
This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene or pol gene of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein. Also provided is a method for transferring a gene of interest into a mammalian cell.
1. A BIV vector construct comprising:
a) a DNA segment from a BIV genome;
b) a packaging sequence to package RNA into virions;
c) a first promoter operably linked to the DNA segment; and
d) a transgene operably linked to a second promoter, wherein at least one of vpw, vpy and tat is deleted from said construct.
2. The BIV vector construct of claim 1 wherein the packaging sequence is a BIV packaging sequence.
3. The BIV vector construct of claim 1 wherein said first promoter is an LTR promoter.
4. The BIV vector construct of claim 1 wherein said first promoter is a CMV promoter.
5. The BIV vector construct of claim 1 wherein the transgene is operably linked to a CMV promoter, a PGK promoter, or an MND promoter.
6. The BIV vector construct of claim 1 wherein the DNA segment further comprises a portion of a gag gene.
7. The BIV vector construct of claim 1 further comprising a rev-response element (RRE).
8. The BIV vector construct of claim 1 further comprising a human interferon-beta scaffold attachment region (SAR).
9. The BIV vector construct of claim 1 further comprising a central polypurine tract (cPPT).
10. A vector construct comprising:
a promoter linked to a first BIV R region;
a BIV U5 element linked to the first BIV R region;
a packaging sequence;
a transgene; and
a BIV U3 element linked to a second BIV R region, wherein the promoter initiates RNA transcription of the vector construct, and at least one of vpw, vpy and tat is deleted from said construct.
11. The vector construct of claim 10 , wherein the transgene is operably linked to an internal promoter.
12. The vector construct of claim 10 , wherein one or more nucleotides in the U3 element are mutated or deleted to diminish or eliminate U3-mediated transcription.
13. The vector construct of claim 10 , wherein the packaging sequence is a BIV packaging sequence.
14. The vector construct of claim 10 , wherein any start codons in the packaging sequence are eliminated by deletion or mutation.
15. The vector construct of claim 10 , wherein the U3 element further comprises a sequence that enhances polyadenylation.
16. The vector construct of claim 10 , further comprising a cPPT.
17. The vector construct of claim 16 , wherein the cPPT is a BIV cPPT.
18. The vector construct of claim 10 , further comprising a 3′ polypurine tract.
19. The vector construct of claim 10 , further comprising an RNA transport element.
20. The vector construct of claim 19 , wherein the RNA transport element is a lentiviral rev response element (RRE).
21. The vector construct of claim 20 , wherein the lentiviral RRE is a BIV RRE.
22. The vector construct of claim 19 , wherein the RNA transport element is a constitutive transport element (CTE).
23. The vector construct of claim 22 , wherein the CTE is a Mason-Pfizer Monkey Virus CTE.
24. A cell comprising a BIV vector construct comprising:
a) a DNA segment from a BIV genome,
b) a packaging sequence to package RNA into virions,
c) a first promoter operably linked to the DNA segment, and
d) a transgene operably linked to a second promoter, wherein at least one of vpw, vpy and tat is deleted from said construct.
25. The cell of claim 24 , wherein said packaging sequence is a BIV packaging sequence.
26. The cell of claim 24 , wherein said first promoter is an LTR promoter.
27. The cell of claim 24 , wherein said first promoter is a CMV promoter.
28. The cell of claim 24 , wherein said transgene is operably linked to a CMV promoter, a PGK promoter or an MND promoter.
29. The cell of claim 24 , wherein said DNA segment further comprises a portion of a gag gene.
30. The cell of claim 24 , wherein said construct further comprises a rev-response element (RRE).
31. The cell of claim 30 , wherein said RRE is a BIV RRE.
32. The cell of claim 24 , wherein said construct further comprises a human interferon-beta scaffold attachment region (SAR).
33. The cell of claim 24 , wherein said construct further comprises a central polypurine tract (cPPT).
34. The cell of claim 24 , wherein said construct comprises a portion of a U3 region of a BIV 3′ LTR.
35. The cell of claim 34 , wherein a portion of said U3 region of a 3′ LTR is deleted or replaced by a heterologous sequence.
36. A cell comprising a vector construct comprising:
a promoter linked to a first BIV R region;
a BIV U5 element linked to the first BIV R region;
a packaging sequence;
a transgene; and
a BIV U3 element linked to a second BIV R region, wherein the promoter initiates RNA transcription of the vector construct, and at least one of vpw, vpy and tat is deleted from said construct.
37. The cell of claim 36 , wherein said transgene is operably linked to an internal promoter.
38. The cell of claim 36 , wherein one or more nucleotides in said U3 element are mutated or deleted to diminish or eliminate U3-mediated transcription.
39. The cell of claim 36 , wherein said packaging sequence is a BIV packaging sequence.
40. The cell of claim 36 wherein any start codons in said packaging sequence are eliminated by deletion or mutation.
41. The cell of claim 36 , wherein said U3 element further comprises a sequence that enhances polyadenylation.
42. The cell of claim 36 , wherein said construct further comprises a major splice donor site.
43. The cell of claim 42 , wherein said major splice donor site has been inactivated or eliminated.
44. The cell of claim 36 , wherein said vector further comprises a cPPT.
45. The cell of claim 44 , wherein said cPPT is a BIV cPPT.
46. The cell of claim 36 , wherein said vector further comprises a 3′ polypurine tract.
47. The cell of claim 36 , wherein said vector further comprises an RNA transport element.
48. The cell of claim 47 , wherein said RNA transport element is a lentiviral rev response element (RRE).
49. The cell of claim 48 , wherein said lentiviral RRE is a BIV RRE.
50. The cell of claim 47 , wherein said RNA transport element is a constitutive transport element (CTE).
51. The cell of claim 50 , wherein said CTE is a Mason-Pfizer Monkey Virus CTE.
52. The BIV vector construct of claim 7 , wherein said RRE is a BIV RRE.
53. The BIV vector construct of claim 1 , further comprising a portion of a U3 region of a BIV 3′ LTR.
54. The vector construct of claim 10 , further comprising a major splice donor site.
55. The BIV vector construct of claim 53 , wherein a portion of the U3 region of the 3′ LTR is deleted or replaced by a heterologous sequence.
56. The vector construct of claim 54 , wherein the major splice donor site has been inactivated or eliminated.