System for pH-neutral stable electrophoresis gel
A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH.
1. A continuous electrophoresis system, comprising an electrophoresis gel comprising a gel buffer and polyacrylamide, wherein the gel buffer has a pH from 6 to 8 and comprises N-tris(hydroxymethyl)methylglycine.
2. The continuous electrophoresis system of claim 1 , wherein the gel buffer has a pH from 6.5 to 7.5.
3. The continuous electrophoresis system of claim 1 , wherein the gel buffer further comprises sodium dodecyl sulfate.
4. The continuous electrophoresis system of claim 1 , wherein the gel buffer comprises 50 mM to 0.45 M N-tris(hydroxymethyl)methylglycine.
5. The continuous electrophoresis system of claim 1 , wherein the gel buffer comprises a Tris salt of a zwitterionic compound.
6. The continuous electrophoresis system of claim 5 , wherein the zwitterionic compound is N-tris(hydroxymethyl)methylglycine.
7. The continuous electrophoresis system of claim 6 , wherein the gel buffer further comprises 50 mM to 0.45 M N-tris(hydroxymethyl)methylglycine.
8. The continuous electrophoresis system of claim 1 , further comprising a sample buffer comprising sodium bisulfite, thioglycolic acid, dithiothreitol, or beta-mercaptoethanol.
9. The continuous electrophoresis system of claim 1 , further comprising a sample buffer comprising a dye.
10. The continuous electrophoresis system of claim 1 , wherein the system is capable of separating a protein having a molecular weight from 2 to 200 kDa.
11. The continuous electrophoresis system of claim 1 , wherein the gel comprises 3% to 25% acrylamide.
12. A method for performing electrophoresis to separate a protein, the method comprising:
providing a continuous electrophoresis system comprising a sample buffer and an electrophoresis gel, wherein the electrophoresis gel comprises a gel buffer and polyacrylamide, wherein the gel buffer has a pH from 6 to 8 and comprises N tris(hydroxymethyl)methylglycine; and
separating the protein using the continuous electrophoresis system by loading a sample comprising the protein into the electrophoresis system and applying an electrical field through the gel.
13. The method of claim 12 , wherein the gel buffer comprises 50 mM to 0.45 M N-tris(hydroxymethyl)methylglycine.
14. The method of claim 12 , wherein the gel buffer comprises a Tris salt of a zwitterionic compound.
15. The method of claim 14 , wherein the zwitterionic compound is N-tris(hydroxymethyl)methylglycine.
16. The method of claim 12 , wherein the sample buffer comprises sodium bisulfate, thioglycolic acid, dithiothreitol, or beta-mercaptoethanol.
17. The method of claim 12 , wherein the sample buffer comprises a dye.
18. The method of claim 12 , wherein the gel buffer comprises sodium dodecyl sulfate.
19. The method of claim 12 , wherein the protein that is separated has a molecular weight from 2 to 200 kDa.
20. The method of claim 12 , wherein the gel of the continuous electrophoresis system comprises 3% to 25% acrylamide.