Nematode ATP synthase subunit E-like sequences
Nucleic acid molecules from nematodes encoding ATP synthase subunit E polypeptides are described. ATP synthase subunit E-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of ATP synthase subunit E-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
1 - 42 . (canceled)
43 . A method comprising:
(a) providing a polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E, the polypeptide having ATP synthase subunit E activity;
(b) contacting the polypeptide with a test compound; and
(c) measuring the binding of the test compound to the polypeptide.
44 . The method of claim 43 , further comprising:
(d) measuring the ATP synthase subunit E activity of the polypeptide in the presence of the test compound.
45 . The method of claim 43 , further comprising:
(d) providing a second polypeptide, wherein the second polypeptide comprises the amino acid sequence of a mammalian or plant ATP synthase subunit E and has ATP synthase subunit E activity;
(e) contacting the second polypeptide with a test compound; and
(f) measuring the binding of the test compound to the second polypeptide.
46 . A method comprising:
(a) providing a polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E, the polypeptide having ATP synthase subunit E activity;
(b) contacting the polypeptide with a test compound; and
(c) measuring a ATP synthase subunit E activity of the polypeptide, wherein a change in ATP synthase subunit E activity of the polypeptide in the presence of the test compound relative to the ATP synthase subunit E activity of the polypeptide in the absence of the test compound is an indication that the test compound alters the ATP synthase subunit E activity of the polypeptide.
47 . The method of claim 46 , further comprising:
(d) providing a second polypeptide, wherein the second polypeptide comprises the amino acid sequence of a mammalian or ATP synthase subunit E and has ATP synthase subunit E activity;
(e) contacting the second polypeptide with the test compound; and
(f) measuring a ATP synthase subunit E activity of the second polypeptide in the presence of the test compound.
48 . The method of claim 43 or 46 wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO: 4.
49 . The method of claim 48 wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO: 4.
50 . The method of claim 48 wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 4.
51 . The method of claim 48 wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 4.
52 . The method of claim 43 or 46 wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 5, and 6.