Collagen-binding proteins from enterococcal bacteria
View Patent ↗A collagen-binding MSCRAMM entitled Ace from enterococcal bacteria is provided which was homologous to the ligand-binding region of Cna, the collagen-binding MSCRAMM from Staphylococcus aureus , and which can be utilized to inhibit adhesion of enterococcal bacteria to extracellular matrix proteins. The N-terminal region of Ace contained a region (residues 174-319), or A domain, contains several 47-residue tandem repeat units between the collagen-binding site and cell wall-associated regions. The Ace protein can be utilized in methods of preventing and/or treating enterococcal infection, and in addition, antibodies raised against Ace, or its A domain, can be used to effectively inhibit the adhesion of enterococcal cells to a collagen substrate.
1. An isolated collagen-binding protein from Enterococcus faecalis wherein the protein has the amino acid sequence of SEQ ID NO:1.
2. The isolated protein according to claim 1 wherein the protein is encoded by a nucleic acid molecule having the sequence of SEQ ID NO.2.
3. The isolated protein according to claim 1 wherein the collagen-binding protein is a protein that binds to a collagen selected from the group consisting of collagen type I, and collagen type IV.
4. A pharmaceutical composition comprising the collagen-binding protein of claim 1 and a pharmaceutically acceptable vehicle, carrier or excipient.
5. An isolated collagen-binding peptide from the collagen-binding domain of a collagen-binding protein comprising amino acids 174-319 from SEQ ID NO:1.
6. A pharmaceutical composition comprising the isolated peptide according to claim 5 and a pharmaceutically acceptable vehicle, carrier or excipient.