Method and system for solubilizing protein
View Patent ↗A process for solubilization of protein including application of an alkali, such as lime, and heating. The process may also involve lime recovery and may be accomplished in a single stage or two stages to separate protein solubilized from labile and recalcitrant sources. Systems and devices for use in such process, including a continuous stirred tank reactor and a plug flow reactor are also involved.
1. A recirculating method of solubilizing protein and destroying prions comprising the following steps in the following order:
first, applying an alkali to an untreated protein source to form a slurry, wherein the alkali is added in an amount sufficient to induce hydrolysis of the protein source when the slurry is heated;
second, heating the slurry to a temperature sufficient to allow hydrolysis of protein in the protein source to obtain a reaction liquid comprising small peptides and free amino acids, and further comprising solid residues;
third, holding the reaction liquid at an elevated temperature between 125-250° C. for a time period sufficient to destroy prions in the reaction liquid;
fourth, separating the solid residues from the reaction liquid;
fifth, neutralizing the reaction liquid with acid or an acid source to produce a neutralized liquid comprising small peptides and free amino acids;
sixth, concentrating the neutralized liquid to produce concentrated liquid comprising small peptides and free amino acids and water; and
seventh, returning the water to the slurry before or during the heating step.
2. The method according to claim 1 , further comprising grinding the protein source.
3. The method according to claim 1 , wherein the alkali comprises calcium oxide.
4. The method according to claim 1 , wherein the alkali comprises a compound selected from the group consisting of: magnesium oxide, magnesium hydroxide, sodium hydroxide, sodium carbonate, potassium hydroxide, ammonia, and any combinations thereof.
5. The method according to claim 1 , further comprising neutralizing the ammonia produced during the heating of the slurry with an acid.
6. The method according to claim 1 , further comprising separating reactive solid residues from inert solid residues in the separated solid residues.
7. The method according to claim 1 , wherein the time period that the reaction liquid is held at an elevated temperature sufficient to destroy prions is between 1 second and 5 hours.
8. The method according to claim 1 , further comprising separating solid residues from the neutralized liquid.
9. The method according to claim 1 , wherein concentrating the neutralized liquid further comprises evaporating the neutralized liquid.
10. The method according to claim 1 , wherein concentrating the neutralized liquid further comprises filtering the neutralized liquid.
11. The method according to claim 1 , wherein concentrating the neutralized liquid further comprises freezing the neutralized liquid.
12. The method according to claim 1 , wherein concentrating the neutralized liquid further comprises adding an immiscible amine and extracting water using the immiscible ammine.
13. The method according to claim 1 , further comprising drying the concentrated liquid.
14. The method according to claim 1 , further comprising producing process heat and reusing process heat.
15. The method according to claim 1 , wherein the alkali comprises calcium hydroxide.
16. The recirculating method of solubilizing protein comprising the following steps in the following order:
first, applying an alkali to a protein source to form a slurry, wherein the alkali is added in an amount sufficient to induce hydrolysis of the protein source when the slurry is heated;
second, heating the slurry to a temperature sufficient to allow hydrolysis of protein in the protein source to obtain a reaction liquid comprising solid residues;
third, separating the solid residues from the reaction liquid;
fourth, neutralizing the reaction liquid with acid or an acid source to produce a neutralized liquid;
fifth, concentrating the neutralized liquid to produce concentrated liquid and water; and
sixth, returning the water to the slurry before or during the heating step.
17. The method according to claim 16 , further comprising grinding the protein source.
18. The method according to claim 16 , wherein the alkali comprises calcium oxide.
19. The method according to claim 16 , wherein the alkali comprises a compound selected from the group consisting of: magnesium oxide, magnesium hydroxide, sodium hydroxide, sodium carbonate, potassium hydroxide, ammonia, and any combinations thereof.
20. The method according to claim 16 , further comprising neutralizing the ammonia produced during the heating of the slurry with an acid.
21. The method according to claim 16 , further comprising returning separated solid residues to the protein source.
22. The method according to claim 16 , further comprising separating reactive solid residues from inert-solid residues in the separated solid residues.
23. The method according to claim 16 , further comprising separating solid residues from the neutralized liquid.
24. The method according to claim 23 , wherein the solid residues separated from the neutralized liquid comprise the alkali and further comprising adding the separated solid residues to the protein source.
25. The method according to claim 16 , wherein concentrating the neutralized liquid further comprises evaporating the neutralized liquid.
26. The method according to claim 16 , wherein concentrating the neutralized liquid further comprises filtering the neutralized liquid.
27. The method according to claim 16 , wherein concentrating the neutralized liquid further comprises freezing the neutralized liquid.
28. The method according to claim 16 , wherein concentrating the neutralized liquid further comprises adding an immiscible amine and extracting water using the immiscible amine.
29. The method according to claim 16 , further comprising drying the concentrated liquid.
30. The method according to claim 16 , further comprising producing process heat and reusing process heat.
31. The method according to claim 16 , wherein the alkali comprises calcium hydroxide.