Method of treating a cancer
View Patent ↗The present invention provides isolated nucleic acids encoding delta DNA methyltransferase 3B molecules that are involved in the treatment and prevention of cancers such as, but not limited to, lung cancer. The delta DNA methyltransferase 3B molecules of the present invention are found to play a critical role in promoter-specific methylation of tumor suppressor genes. The DNA methyltransferase 3B molecules of the present invention are provided as therapeutic targets for identifying inhibitors of DNA methyltransferase. Such inhibitors are contemplated for the treatment and/or prevention of cancers. In particular embodiments, the present invention involves the treatment and prevention of a non-small cell lung cancer.
1. An isolated nucleic acid sequence encoding a DNA methyltransferase-3B variant having the amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18.
2. The nucleic acid sequence of claim 1 , comprising a DNA methyltransferase-3B variant nucleic acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4.
3. The isolated nucleic acid of claim 2 , wherein the nucleic acid sequence is comprised in an expression vector.
4. The isolated nucleic acid of claim 3 , wherein the expression vector is further defined as a viral or plasmid vector.
5. The isolated nucleic acid of claim 4 , wherein the viral vector is an adenoviral vector, an adeno-associated viral vector, a retroviral vector, a lentiviral vector, a herpes viral vector, polyoma viral vector or hepatitis B viral vector.
6. The isolated nucleic acid sequence of claim 3 , wherein the expression vector is comprised in a non-viral delivery system.
7. The isolated nucleic acid sequence of claim 6 , wherein the non-viral delivery system comprises one or more lipids.
8. The isolated nucleic acid sequence of claim 2 , wherein the nucleic acid sequence is operatively linked to a promoter.
9. A host cell comprising a nucleic acid sequence encoding a DNA methyltransferase-3B variant according to claim 2 .
10. The host cell of claim 9 , wherein the nucleic acid sequence is comprised in a vector.
11. An isolated DNA methyltransferase-3B variant having the amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18.
12. The nucleic acid sequence of claim 2 , wherein said sequence is SEQ ID NO:2.
13. The nucleic acid sequence of claim 2 , wherein said sequence is SEQ ID NO:3.
14. The nucleic acid sequence of claim 2 , wherein said sequence is SEQ ID NO:4.
15. The nucleic acid sequence of claim 1 , encoding a DNA methyltransferase-3B variant having the amino acid sequence of SEQ ID NO:16.
16. The nucleic acid sequence of claim 1 , encoding a DNA methyltransferase-3B variant having the amino acid sequence of SEQ ID NO:17.
17. The nucleic acid sequence of claim 1 , encoding a DNA methyltransferase-3B variant having the amino acid sequence of SEQ ID NO:18.
18. The DNA methyltransferase-3B variant of claim 11 , having the amino acid sequence of SEQ ID NO:16.
19. The DNA methyltransferase-3B variant of claim 11 , having the amino acid sequence of SEQ ID NO:17.
20. The DNA methyltransferase-3B variant of claim 11 , having the amino acid sequence of SEQ ID NO:18.