Comparative phenotype analysis of cells including testing of biologically active chemicals
The present invention relates to growing and testing any cell type in a multitest format. The present invention is suited for the characterization of microorganisms, as well as animal and plant cells. The present invention is also particularly suited for analysis of phenotypic differences between strains of organisms, including cultures that have been designated as the same genus and species. The present invention is also suited for the analysis of phenotypic differences between cell lines. In some embodiments, a gel forming matrix is used. The present invention provides methods and compositions for the phenotypic analysis and comparison of eukaryotic, as well as prokaryotic cells. The present invention further provides novel methods and compositions for testing the effect(s) of biologically active chemicals on various cells.
1 . A method for testing animal or plant cells, comprising the steps of:
a) providing a testing device comprising a plurality of testing wells, wherein said testing wells contain at least one testing substrate selected from the group consisting of carbon sources, nitrogen sources, phosphorus sources, sulfur sources, biologically active chemicals, and chromogenic compounds;
b) preparing a suspension comprising a pure culture of cells in a suspension medium;
c) introducing said suspension into said testing wells of said testing device; and
d) observing at least one response of said cells to said testing substrate.