Compositions and methods useful for the diagnosis and treatment of heparin induced thrombocytopenia/thrombosis
The invention includes compositions, kits and methods comprising a monoclonal antibody which shares key functional properties with the polyclonal antibodies which participate in the pathogenesis of heparin induced thrombocytopenia/thrombosis (HIT/HITT) in a mammal. The monoclonal antibody of the invention preferentially binds with a PF4/heparin complex relative to the binding of the antibody with PF4 or heparin alone. The monoclonal antibody of the invention also binds specifically with PF4 in a complex with other glycosaminoglycans besides heparin, and also activates platelets. The monoclonal antibody of the invention is useful in methods for diagnosing and treating HIT/HITT in a mammal. A humanized version of the monoclonal antibody of the invention is also included, along with a process for humanizing the monoclonal antibody of the invention.
1. A Fab antibody capable of binding specifically with a platelet factor 4 (PF4)/heparin complex, wherein said antibody preferentially binds with said PF4/heparin complex relative to the binding of said antibody with either PF4 or heparin alone, wherein the Fab antibody comprises an antibody-binding fragment comprising the amino acid sequence of SEQ ID NO: 11, and wherein the Fab antibody further comprises the amino acid sequence of SEQ ID NO:13.
2. A kit for diagnosing HIT/HITT in a mammal, said kit comprising:
(a) a positive control solution comprising a monoclonal antibody or antigen-binding fragment thereof which binds specifically with a platelet factor 4 (PF4)/heparin complex, wherein said monoclonal antibody or antigen-binding fragment thereof preferentially binds with said PF4/heparin complex relative to the binding of said monoclonal antibody or antigen-binding fragment thereof with either PF4 or heparin alone, and wherein the monoclonal antibody or antigen-binding fragment thereof comprises the amino acid sequence of SEQ ID NO: 11 and the amino acid sequence of SEQ ID NO:13; and
(b) an instructional material describing the use of said positive control solution for diagnosing HIT/HITT in a mammal.
3. The kit of claim 2 wherein the monoclonal antibody is produced by hybridoma KKO deposited under ATCC Accession No: PTA-6133.
4. A kit for diagnosing HIT/HITT in a mammal, said kit comprising:
(a) a positive control solution comprising a monoclonal antibody or an antigen-binding fragment thereof which binds specifically with a platelet factor 4 (PF4)/heparin complex, wherein said monoclonal antibody or antigen-binding fragment thereof preferentially binds with said PF4/heparin complex relative to the binding of said monoclonal antibody or antigen-binding fragment thereof with either PF4 or heparin alone, and wherein the monoclonal antibody is produced by hybridoma KKO deposited under ATCC Accession No: PTA-6133; and
(b) an instructional material describing the use of said positive control solution for diagnosing HIT/HITT in a mammal.