Amyloid plaque aggregation inhibitors and diagnostic imaging agents
View Patent ↗This invention relates to a method of imaging amyloid deposits and to labeled compounds, and methods of making labeled compounds useful in imaging amyloid deposits. This invention also relates to compounds, and methods of making compounds for inhibiting the aggregation of amyloid proteins to form amyloid deposits, and a method of delivering a therapeutic agent to amyloid deposits.
1. A pharmaceutical composition, comprising a compound of Formula III and a pharmaceutically acceptable excipient or diluent, wherein a compound of Formula III is selected from:
or a pharmaceutically acceptable salt thereof, wherein
R 3 is hydrogen or -L-Ch;
R 1 and R 2 are independently hydrogen, C 1-4 alkyl, C 2-4 aminoalkyl, C 1-4 haloalkyl, haloarylalkyl, -L-Ch, or R 1 and R 2 are taken together with the nitrogen to which they are attached to form a 5-to 7-member heterocyclic ring optionally having O,S or NR 5 in said ring, where
R 5 is hydrogen or C 1-4 alkyl;
L is a covalent bond or linking group selected from the group consisting of: —(CH 2 ) n —, or —(CH 2 ) n —C(O)— where
n is an integer from 0 to 5; and
Ch is a tetradentate ligand capable of complexing with a metal;
with the proviso that one and only one of R 1 , R 2 and R 3 is -L-Ch.
2. The pharmaceutical composition of claim 1 , wherein n is an integer from 0 to 3.
3. The pharmaceutical composition of claim 1 , wherein R 3 is -L-Ch.
4. The pharmaceutical composition of claim 1 , wherein Ch is selected from the group consisting of:
5. The pharmaceutical composition of claim 4 , wherein n is 0.
6. The pharmaceutical composition of claim 3 , wherein Ch is:
7. The pharmaceutical composition of claim 6 , wherein R 1 and R 2 are each independently hydrogen or C 1-4 alkyl.
8. The pharmaceutical composition of claim 7 , comprising a compound having the following structure:
9. The pharmaceutical composition of claim 1 , wherein said metal is technetium or rhenium.
10. The pharmaceutical composition of claim 1 , wherein one of R 1 and R 2 is -L-Ch.
11. The pharmaceutical composition of claim 1 , wherein R 1 and R 2 are independently hydrogen or C 1-4 alkyl.
12. The pharmaceutical composition of claim 1 , wherein R 1 and R 2 are both methyl.
13. A diagnostic composition for imaging amyloid deposits, comprising a radiolabeled compound of claim 1 and a pharmaceutically acceptable excipient or diluent.
14. A method of decreasing amyloid plaque aggregation in a mammal comprising administering a composition of claim 1 in an amount effective to decrease amyloid plaque aggregation.
15. A method of imaging amyloid deposits, comprising:
a. introducing into a mammal a detectable quantity of a diagnostic composition of claim 13 ; and
b. allowing sufficient time for the labeled compound to become associated with amyloid deposits; and
c. detecting the labeled compound.
16. A method of preparing a compound of claim 3 comprising,
a. contacting a compound having the structure:
wherein —Ch is selected from the group consisting of:
wherein R 9 is hydrogen or a sulfur protecting group, with a reducing agent to prepare a mixture; and
b. contacting said mixture with a composition comprising [ 99m Tc]Pertechnate;
wherein a compound having the following structure:
wherein —Ch is selected from the group consisting of:
is prepared.
17. The method of claim 16 , wherein n is 0.
18. The method of claim 17 , wherein a compound having the following structure:
is prepared.
19. The pharmaceutical composition of claim 1 , wherein Ch is selected from the group consisting of:
wherein R 9 is hydrogen or a sulfur protecting group.
20. A diagnostic kit comprising:
(a) a composition comprising a non-radiolabeled compound of claim 1 ; and
(b) a reducing agent, and optionally, (c) a chelator.