Isolated plant deoxyhypusine synthase and nucleotides encoding same
Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced eIF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.
1 - 70 . (canceled)
71 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of C-K-X 1 -F-L-G-F-T-S-N-L-X 2 -S-S-G-V-R, wherein X 1 and X 2 are independently either an I or V, and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
72 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 71 .
73 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of N-R-I-G-N and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
74 . A isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 73 .
75 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of F-T-S-N L and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
76 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 75 .
77 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of G-F-Q-A and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
78 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 77 .
79 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of V-T-T-X 1 -G-G X 2 -E-E-D wherein X 1 is A or T and X 2 is I or V and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
80 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 79 .
81 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of I-L-G-G-G-L-P-K-H-H and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
82 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 81 .
83 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of R-N-G-A-D-X,-A-V, wherein X 1 is Y or F and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
84 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 83 .
85 . An isolated plant deoxyhypusine synthase comprising the amino acid motif of E-F-D-G-S-D-S-G-A-R-P-D-E-A-X 1 -S-W-G-K, wherein X 1 is V or I and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.
86 . An isolated polynucleotide encoding the isolated deoxyhypusine synthase of claim 85 .
87 . An isolated plant deoxyhypusine synthase claim 85 comprising the amino acid motif of E-F-D-G-S-D-S-G-A-R-P-D-E-A-X 1 -S-W-G, wherein X 1 is V or I and wherein the isolated deoxyhypusine synthase exhibits deoxyhypusine synthase catalytic activity.