D-isomers of antimicrobial peptide
View Patent ↗This invention provides D-isomers of MUC7-12-mer peptide of human saliva MUC7. The isomers have antimicrobial activity comparable to that of the L-isomers and are resistant to proteolysis. These peptides can be used as antifungal and antimicrobial agents.
1. An isolated and purified peptide of SEQ ID NO:1, wherein all of the amino acids are D amino acids.
2. A method of killing bacteria or fungi comprising the steps of exposing the bacterial or fungal cells to a bactericidal or fungicidal composition comprising the peptide of claim 1 .
3. The method of claim 2 , wherein the bacteria are selected from the group consisting of, Escherichia coli, Streptoccocus gordonii, Streptococcus mutans, Actinobacillus actinomycetemcimitans and Pseudomonas gingivalis.
4. The method of claim 2 , wherein the bacteria are present in a biofilm.
5. The method of claim 4 , wherein the biofilm is present in the oral cavity.
6. The method of claim 5 , wherein the bacteria in the biofilm comprise S. mutans.
7. The method of claim 2 , wherein the fungi are selected from the group consisting of Candida albicans, Candida glabrata, Crytococus neoformans, Candida krusei and Saccharomyces cerevisiae.
8. The method of claim 2 , wherein the peptide is used at a concentration of 25 to 200 μM.
9. The method of claim 8 , wherein the peptide is used at a concentration of about 100 μM.
10. The method of claim 9 , wherein the peptide is used with EDTA at a concentration of about 1 mM.
11. An oral composition comprising the peptide of claim 1 .
12. The oral composition of claim 11 further comprising ethylenediaminetratra acetic acid (EDTA).
13. The oral composition of claim 12 , wherein the EDTA is present at a concentration of between 0.5 to 2.0 mM.
14. The oral composition of claim 13 , wherein the EDTA is present at a concentration of about 1 mM.