C-C chemokine receptor 3 proteins
The present invention relates to isolated and/or recombinant C-C Chemokine Receptor 3 (CKR-3, CCR3) proteins or polypeptides.
1. An isolated polypeptide comprising a mammalian C-C chemokine receptor 3 protein (CCR3), wherein said CCR3 has at least about 95% amino acid sequence identity with SEQ ID NO:2 or SEQ ID NO:6, and said CCR3 binds a ligand selected from the group consisting of RANTES and MCP-3.
2. The isolated polypeptide of claim 1 , wherein said CCR3 mediates signaling, a cellular response, or signaling and a cellular response upon binding said ligand, wherein said signaling is activation of a G protein or a rapid and transient increase in the concentration of cytosolic free calcium ([Ca 2+ ] i ), and said cellular response is chemotaxis, exocytosis, inflammatory mediator release or integrin activation.
3. The isolated polypeptide of claim 1 , wherein the amino acid sequence of said CCR3 is SEQ ID NO:2.
4. The isolated polypeptide of claim 1 , wherein the amino acid sequence of said CCR3 is SEQ ID NO:6.
5. The isolated polypeptide of claim 1 , wherein said isolated polypeptide is a fusion protein.
6. An isolated polypeptide comprising a mammalian C-C chemokine receptor 3 protein (CCR3), wherein said CCR3 has at least about 95% amino acid sequence identity with SEQ ID NO:2 or SEQ ID NO:6, and said CCR3 binds eotaxin.
7. The isolated polypeptide of claim 6 , wherein said CCR3 mediates signaling, a cellular response, or signaling and a cellular response upon binding eotaxin, wherein said signaling is activation of a G protein or a rapid and transient increase in the concentration of cytosolic free calcium ([Ca 2+ ] i ), and said cellular response is chemotaxis, exocytosis, inflammatory mediator release or integrin activation.
8. The isolated polypeptide of claim 6 , wherein the amino acid sequence of said CCR3 is SEQ ID NO:2.
9. The isolated polypeptide of claim 6 , wherein the amino acid sequence of said CCR3 is SEQ ID NO:6.
10. The isolated polypeptide of claim 6 , wherein said isolated polypeptide is a fusion protein.
11. An isolated polypeptide comprising a C-C chemokine receptor 3 protein (CCR3), wherein the amino acid sequence of said CCR3 protein is selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:6.
12. The isolated polypeptide comprising a C-C chemokine receptor 3 protein (CCR3) of claim 11 , wherein the amino acid sequence of said CCR3 protein consists of SEQ ID NO:2.
13. An isolated polypeptide comprising a mammalian C-C chemokine receptor 3 protein (CCR3) encoded by SEQ ID NO:1.
14. An isolated polypeptide comprising a mammalian C-C chemokine receptor 3 protein (CCR3) encoded by SEQ ID NO:5.
15. The isolated polypeptide of claim 13 , wherein said isolated polypeptide is a fusion protein.
16. The isolated polypeptide of claim 14 , wherein said isolated polypeptide is a fusion protein.