Taxoid oxygenases and their uses
View Patent ↗Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
1. A plant cell transformed with a nucleic acid molecule comprising a nucleic acid sequence having at least 95% sequence identity with SEQ ID NO:82; wherein the nucleic acid sequence encodes a polypeptide having taxoid oxygenase activity.
2. The plant cell of claim 1 , wherein the nucleic acid molecule further comprises a promoter sequence operably linked to the nucleic acid sequence.
3. The plant cell of claim 2 , wherein the nucleic acid molecule comprises the nucleic acid sequence of SEQ ID NO:82.
4. A plant cell transformed with a nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity with SEQ ID NO: 88; wherein the polypeptide has taxoid oxygenase activity.
5. The plant cell of claim 4 , wherein the nucleic acid sequence encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:88.
6. The plant cell of claim 4 , wherein the nucleic acid molecule further comprises a promoter sequence operably linked to the nucleic acid sequence.
7. A method for producing a taxoid oxygenase, comprising growing a cell transformed with a nucleic acid molecule comprising a nucleic acid sequence having at least 95% sequence identity with SEQ ID NO:82, wherein the nucleic acid sequence encodes a polypeptide having taxoid oxygenase activity, and recovering from the cell the taxoid oxygenase encoded by the nucleic acid sequence.
8. A method for producing a taxoid oxygenase, comprising growing the plant cell of claim 4 and recovering from the plant cell the taxoid oxygenase encoded by the nucleic acid sequence.