The present invention provides a vector which can make nucleic acids to be an aggregate and locate a vector containing a cationic polymer to surround the aggregate so as to protect the nucleic acids from the enzyme. The vector comprises a polymer having branched chain(s). Preferably, 3, 4, or 6 branched chains are bonded to benzene ring. It is preferable that the number of the branched chains is higher. As the branched chain(s), a vinyl-series acrylic polymer is preferable. The vector is synthesized by reacting a dithiocarbamate compound with an acrylamide monomer for the branched chain(s).
1. A nucleic-acid-containing complex comprising a nucleic acid and a vector, wherein the vector is represented by a Formula selected from the group consisting of Formula (I) and Formula (II):
wherein R 1 -R 6 each represent polymer chains, wherein R 1 -R 6 may each be the same or different,
wherein R 7 -R 10 each represent polymer chains, wherein R 7 -R 10 may each be the same or different,
wherein the polymer chains represented by R 1 -R 10 are polymers consisting of dimethylaminopropyl acrylamide monomers, and
wherein said nucleic-acid-containing complex has a particle size of about 50 to about 400 nm.
2. The nucleic-acid-containing complex of claim 1 , wherein the vector is of Formula II and has a molecular weight of from 30,000 to 65,000.
3. The nucleic-acid-containing complex of claim 1 , wherein the vector is of Formula I and has a molecular weight of from 10,000 to 50,000.
4. A nucleic-acid-containing complex as claimed in claim 1 , wherein said vector is represented by Formula (I).
5. The nucleic-acid-containing complex of claim 1 , wherein the molecular weight of the vector is from 5,000 to 500,000.
6. The nucleic-acid-containing complex of claim 1 , wherein the molecular weight of the vector is from 5,000 to 50,000.
7. The nucleic-acid-containing complex as claimed in claim 1 , wherein said nucleic-acid-containing complex has a particle size of approximately 250 nm, wherein a cation/anion ratio of said nucleic-acid-containing complex is 20 to 160, and wherein the vector is represented by Formula (I) and has a molecular weight of approximately 18,000.