IP Library Granted Patent US 8,507,276
Granted Patent B2
US 8,507,276 · App. 11/285,342 · Granted Aug 13, 2013

Tissue culture media used as a component of cosmetics

Inventors: Sven Gohla (Hamburg, DE); Monika Moenks (Schmitten, CH); Sibylle Ibanez (Uetikon am See ZH, CH); Carmen Evangelisti (Freienstein, CH)
Assignee: La Prairie Group AG
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Quick Facts
Patent No.
US 8,507,276
App. No.
11/285,342
Granted
Aug 13, 2013
Kind
B2
Abstract

A cosmetic or dermatological preparation which comprises one or more tissue culture media, in particular one or more skin cell culture media.

Claims (161)

1. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin, comprises one or more tissue culture media selected from HAM's F-10, HAM's F-12, DMEM, and MCDB, further comprises at least one substance selected from sunscreen filters, perfumes, dyes, pearlizing agents, and pigments having a coloring effect, and is present as one of an oil-in-water (O/W) emulsion, a water-in-oil (W/O) emulsion, a water-in-oil-in-water (W/O/W) emulsion, and a microemulsion.

2. The preparation of claim 1 , wherein the preparation comprises one or more tissue culture media selected from HAM's F-10, HAM's F-12, and DMEM.

3. The preparation of claim 1 , wherein the one or more tissue culture media are present in an amount of from 1% to 50% by weight, based on a total weight of the preparation.

4. The preparation of claim 1 , wherein the one or more tissue culture media together comprise all of the following components in the following concentration ranges in mg/l:

Biotin

0.0036

    0.0146

CaCl 2 •2 H 2 O

4.41

116.61

Calcium pantothenate

0.25

  2.24

Choline chloride

9

 13.96

CuSO 4 •5 H 2 O

0.0002496

     0.00125

D-Glucose

1081

3151   

FeSO 4 •7 H 2 O

0.417

   1.39

Folic acid

0.79

   2.65

Glycine

7.51

  18.75

Inositol

12.6

  18.02

KCl

111.83

311.8

L-Alanine

4.5

   8.91

L-Arginine•HCl

147.5

210.7

L-Asparagine

7.5

  15.01

L-Aspartic acid

3.99

   6.65

L-Cysteine•HCl

15.75

 42.04

L-Glutamine

365.3

877.2

L-Glutamic acid

7.35

 14.71

L-Histidine•HCl•H 2 O

16.77

 31.5

Lipoic acid

0.11

     0.2063

L-Isoleucine

1.968

 54.5

L-Leucine

59

 65.6

L-Lysine•HCl

18.27

 91.25

L-Methionine

4.475

 17.24

L-Phenylalanine

4.956

 35.5

L-Proline

17.25

 34.53

L-Serine

26.25

 63.06

L-Threonine

11.91

 53.5

L-Tyrosine

2.718

 38.7

L-Valine

35.13

 52.85

MgCl 2 •6 H 2 O

61

122  

Na 2 HPO 4 •7 H 2 O

71

536.2

NaCl

6999.5

7599  

NaHCO 3

1176

2438  

Sodium pyruvate

47

63

Nicotinamide

0.03663

   2.02

Pyridoxine HCl

0.031

      0.06171

Riboflavin

0.03764

   0.22

Thiamine HCl

0.3373

   2.17

Thymidine

0.37

     0.7266

Vitamin B 12 (cobalamine)

0.68

   4.07.

5. The preparation of claim 1 , wherein the one or more tissue culture media comprise DMEM/HAM's F-12 (1:1).

6. The preparation of claim 1 , which further comprises a mixture comprising collagen, chitosan having a degree of acetylation of up to 50% and glycosylaminoglycan.

7. The preparation of claim 1 , which further comprises one or more serum substitutes.

8. The preparation of claim 7 wherein the one or more serum substitutes function as osmoregulators.

9. The preparation of claim 7 , wherein the one or more serum substitutes comprise one or more of chitosan, chrondroitin-6-sulfate and collagen.

10. The preparation of claim 1 , which further comprises glutamine.

11. The preparation of claim 1 , wherein ratios of ingredients of the one or more cell culture media of mineral and organic biofactors are suitable for the retention, the cultivation and the care of skin cells at least one of in vitro, ex vivo and in vivo.

12. The preparation of claim 1 , wherein the preparation is present as at least one of a spray, a foam, a foam aerosol, an ointment and a cosmetic stick preparation.

13. The preparation of claim 1 , wherein the preparation is present as a O/W emulsion.

14. The preparation of claim 1 , wherein the preparation is present as a W/O emulsion.

15. The preparation of claim 1 , wherein the preparation is present as a W/O/W emulsion.

16. The preparation of claim 1 , wherein the preparation is present as a microemulsion.

17. The preparation of claim 1 , wherein the preparation is associated with instructions directing its use in a cosmetic application.

18. A process for making the preparation of claim 1 , wherein the process comprises mixing the one or more tissue culture media with one or more other cosmetic constituents of the preparation immediately prior to use.

19. The preparation of claim 1 , wherein the preparation is serum-free.

20. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin and comprises one or more tissue culture media, a membrane system, and at least one substance selected from sunscreen filters, perfumes, dyes, pearlizing agents, and pigments having a coloring effect.

21. The preparation of claim 20 , wherein the membrane system comprises a liposome.

22. The preparation of claim 20 , wherein the membrane system comprises a cyclodextrin.

23. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin and comprises one or more tissue culture media and a chitosan/collagen/condroitin 6-sulfate sponge.

24. The preparation of claim 23 , wherein the one or more tissue culture media comprise one or more skin cell culture media.

25. The preparation of claim 24 , wherein the one or more tissue culture media are serum-free.

26. The preparation of claim 23 , which further comprises glutamine.

27. The preparation of claim 23 , wherein the preparation further comprises at least one substance selected from sunscreen filters, perfumes, dyes, pearlizing agents, and pigments with a coloring effect.

28. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin and comprises one or more tissue culture media selected from HAM's F-10, HAM's F-12, DMEM, and MCDB and at least one substance selected from perfumes, and pearlizing agents.

29. The preparation of claim 28 , wherein the preparation is present as at least one of an aqueous or aqueous-alcoholic solution, a spray, a foam, a foam aerosol, an ointment, an aqueous gel and a cosmetic stick preparation.

30. The preparation of claim 28 , wherein the preparation further comprises glutamine.

31. The preparation of claim 28 , wherein the preparation is associated with instructions directing its use in a cosmetic application.

32. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin, comprises one or more tissue culture media selected from HAM's F-10, HAM's F-12, DMEM, and MCDB, further comprises a mixture comprising collagen, chitosan having a degree of acetylation of up to 50%, and glycosylaminoglycan, is serum-free, and is present as one of an oil-in-water (O/W) emulsion, a water-in-oil (W/O) emulsion, a water-in-oil-in-water (W/O/W) emulsion, and a microemulsion.

33. The preparation of claim 32 , which further comprises glutamine.

34. The preparation of claim 32 , wherein the preparation is present as at least one of a spray, a foam, a foam aerosol, an ointment, and a cosmetic stick preparation.

35. A cosmetic or dermatological preparation, wherein the preparation is formulated as a cosmetic or dermatological preparation for application to skin, comprises one or more tissue culture media, at least one of a polyurethane matrix and a chitosan/collagen/condroitin 6-sulfate sponge, and at least one substance selected from sunscreen filters, perfumes, dyes, pearlizing agents, and pigments having a coloring effect.

36. The preparation of claim 35 , wherein the one or more tissue culture media comprise one or more skin cell culture media.

37. The preparation of claim 35 , wherein the one or more tissue culture media are serum-free.

38. The preparation of claim 35 , which further comprises glutamine.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 2, 2019
From: LA PRAIRIE GROUP AG
To: NEC PLUS ULTRA COSMETICS AG
Reel/Frame 049940/0794 →
CHANGE OF NAME Recorded May 3, 2011
From: JUVENA (INTERNATIONAL) AG
To: LA PRAIRIE GROUP AG
Reel/Frame 026218/0874 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 27, 2006
From: GOHLA, SVEN; MOENKS, MONIKA; IBANEZ, SYBILLE; EVANGELISTI, CARMEN
To: JUVENA INTERNATIONAL AG
Reel/Frame 017820/0451 →
Priority Claims (3)
DE 103 23 510 · May 24, 2003 · national
DE 103 55 110 · Nov 24, 2003 · national
DE 10 2004 020 035 · Apr 22, 2004 · national
Continuity (2)
Continuation PCTEP2004005532 · May 22, 2004
Related Publication 20060182701A1 · Aug 17, 2006