T-bet antibodies
Isolated nucleic acid molecules encoding T-bet, and isolated T-bet proteins, are provided. The invention further provides, e.g., anti-T-bet antibodies. Methods of using the T-bet compositions of the invention are also disclosed, including, e.g., methods for detecting T-bet activity in a biological sample.
1. An isolated antibody, or antigen binding fragment thereof, that specifically binds to an epitope of the amino acid sequence of SEQ ID NO:2.
2. An isolated antibody, or antigen binding fragment thereof, that specifically binds to an epitope of a protein encoded by the nucleic acid molecule of SEQ ID NO:1.
3. The antibody, or antigen binding fragment thereof, of claim 1 , which is a polyclonal antibody.
4. The antibody, or antigen binding fragment thereof, of claim 1 , which is a monoclonal antibody.
5. The antibody, or antigen binding fragment thereof, of claim 1 , which is coupled to a detectable substance.
6. The antibody, or antigen binding fragment thereof, of claim 5 , wherein the detectable substance is selected from the group consisting of an enzyme, a fluorescent material, a luminescent material, and a radioactive material.
7. An isolated antibody, or antigen binding fragment thereof, that specifically binds to a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO:2 over its full length, which polypeptide binds a consensus T-box site in DNA and induces IFN-γ production in CD4+ cells, wherein the antibody, or antigen binding fragment thereof, binds to a portion of the polypeptide having at least 90% identity to SEQ ID NO:2.
8. The antibody, or antigen binding fragment thereof, of claim 1 which is an antigen binding fragment of an antibody selected from the group consisting of Fab and F(ab′) 2 fragments.
9. The antibody, or antigen binding fragment thereof, of claim 1 which is a chimeric or humanized antibody.
10. An isolated antibody, or antigen binding fragment thereof, which specifically binds to the amino acid sequence of SEQ ID NO:2 and which is an intracellular antibody.
11. A method for detecting a polypeptide comprising the amino acid sequence of SEQ ID NO:2 in a biological sample, comprising:
contacting the biological sample with the antibody, or antigen binding fragment thereof, of claim 1 , under conditions such that the antibody binds to a polypeptide present in the sample, detecting the polypeptide-antibody complex formed; thereby detecting the presence of the polypeptide in the biological sample.
12. The method of claim 11 , wherein the antibody, or antigen binding fragment thereof, is directly labeled.
13. The method of claim 11 , wherein the antibody, or antigen binding fragment thereof, is indirectly labeled.
14. A method for isolating a polypeptide comprising the amino acid sequence of SEQ ID NO:2 from a biological sample, comprising:
contacting the biological sample with the antibody, or antigen binding fragment thereof, of claim 1 , under conditions such that the antibody binds to a polypeptide present in the sample to form a complex, isolating the polypeptide from the complex, thereby isolating the polypeptide from the biological sample.
15. An isolated antibody, or antigen binding fragment thereof, that specifically binds to an epitope of the amino acid sequence of SEQ ID NO:4.
16. An isolated antibody, or antigen binding fragment thereof, that specifically binds to an epitope of a protein encoded by the nucleic acid molecule of SEQ ID NO:3.
17. An isolated antibody, or antigen binding fragment thereof, that specifically binds to a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO:4 over its full length, which polypeptide binds a consensus T-box site in DNA and induces IFN-γ production in CD4+ cells, wherein the antibody, or antigen binding fragment thereof, binds to a portion of the polypeptide having at least 90% identity to SEQ ID NO:4.
18. A method for detecting a polypeptide comprising the amino acid sequence of SEQ ID NO:4 in a biological sample, comprising:
contacting the biological sample with the antibody, or antigen binding fragment thereof, of claim 15 , or fragment thereof, under conditions such that the antibody binds to a polypeptide present in the sample, detecting the polypeptide-antibody complex formed; thereby detecting the presence of the polypeptide in the biological sample.
19. A method for isolating a polypeptide comprising the amino acid sequence of SEQ ID NO:4 from a biological sample, comprising:
contacting the biological sample with the antibody, or antigen binding fragment thereof, of claim 15 , under conditions such that the antibody binds to a polypeptide present in the sample to form a complex, isolating the polypeptide from the complex, thereby isolating the polypeptide from the biological sample.
20. The antibody, or antigen binding fragment thereof, of claim 2 , which is a polyclonal antibody.
21. The antibody, or antigen binding fragment thereof, of claim 7 , which is a polyclonal antibody.
22. The antibody, or antigen binding fragment thereof, of claim 2 , which is a monoclonal antibody.
23. The antibody, or antigen binding fragment thereof, of claim 7 , which is a monoclonal antibody.
24. The antibody, or antigen binding fragment thereof, of claim 2 , which is coupled to a detectable substance.
25. The antibody, or antigen binding fragment thereof, of claim 7 , which is coupled to a detectable substance.
26. The antibody, or antigen binding fragment thereof, of claim 24 , wherein the detectable substance is selected from the group consisting of an enzyme, a fluorescent material, a luminescent material, and a radioactive material.
27. The antibody, or antigen binding fragment thereof, of claim 25 , wherein the detectable substance is selected from the group consisting of an enzyme, a fluorescent material, a luminescent material, and a radioactive material.
28. The antibody, or antigen binding fragment thereof, of claim 2 , which is an antigen binding fragment of an antibody selected from the group consisting of Fab and F(ab′) 2 fragments.
29. The antibody, or antigen binding fragment thereof, of claim 7 , which is an antigen binding fragment of an antibody selected from the group consisting of Fab and F(ab′) 2 fragments.
30. The antibody, or antigen binding fragment thereof, of claim 2 , which is a chimeric or humanized antibody.
31. The antibody, or antigen binding fragment thereof, of claim 7 , which is a chimeric or humanized antibody.
32. An isolated antibody, or antigen binding fragment thereof, which specifically binds to the amino acid sequence of SEQ ID NO:4 and which is an intracellular antibody.
33. The antibody, or antigen binding fragment thereof, of claim 7 , which is an intracellular antibody.