cGMP-binding, cGMP-specific phosphodiesterase materials and methods
The present invention provides novel purified and isolated nucleotide sequences encoding the cGMP-binding, cGMP-specific phosphodiesterase designated cGB-PDE. Also provided by the invention are methods and materials for the recombinant production of cGB-PDE polypeptide products and methods for identifying compounds which modulate the enzymatic activity of cGB-PDE polypeptides.
1 . A purified and isolated polynucleotide encoding cGB-PDE.
2 . The polynucleotide of claim 1 which is a DNA sequence.
3 . The DNA sequence of claim 2 which is a cDNA sequence or a biological replica thereof.
4 . The DNA sequence of claim 2 which is a genomic DNA sequence or a biological replica thereof.
5 . An RNA transcript of the genomic DNA sequence of claim 4 .
6 . The DNA sequence of claim 2 which is a wholly or partially chemically synthesized DNA sequence or a biological replica thereof.
7 . The DNA sequence of claim 4 further comprising an endogenous expression control DNA sequence.
8 . A DNA vector comprising a DNA sequence according to claim 2 .
9 . The vector of claim 8 wherein said DNA sequence is operatively linked to an expression control DNA sequence.
10 . A host cell stably transformed or transfected with a DNA sequence according to claim 7 in a manner allowing the expression in said host cell of cGB-PDE polypeptide possessing a ligand/receptor binding biological activity or immunological property specific to cGB-PDE.
11 . A method for producing cGB-PDE polypeptide, said method comprising growing a host cell according to claim 10 in a suitable nutrient medium and isolating cGB-PDE polypeptide from said cell or the medium of its growth.
12 . A polypeptide or peptide capable of specifically binding to cGB-PDE.
13 . An antibody substance according to claim 12 .
14 . A monoclonal antibody according to claim 13 .
15 . A hybridoma cell line producing a monoclonal antibody according to claim 14 .
16 . A humanized antibody substance according to claim 13 .
17 . An antisense polynucleotide specific for a polynucleotide encoding cGB-PDE.
18 . A DNA sequence encoding cGB-PDE and selected from the group consisting of:
(a) the DNA sequence set out in SEQ ID NO: 9 or 22;
(b) a DNA which hybridizes under stringent conditions to the DNA of (a); and
(c) a DNA sequence which, but for the redundancy of the genetic code, would hybridize under stringent conditions to a DNA sequence of (a) or (b).