Compositions and methods related to modified retroviral vectors for restricted, site specific integration

1. A retroviral integrase complex comprising:

a) a recombinant retroviral integrase having a domain comprising a non-native protein binding site inserted in an exposed unstructured loop of said integrase; and

b) a DNA binding protein comprising a DNA binding domain and a peptide binding domain that binds the non-native protein binding site of the recombinant integrase.

2. The complex of claim 1 , wherein the retroviral integrase is a FIV integrase, a MLV integrase, or lentivirus integrase.

3. The complex of claim 2 , wherein the retroviral integrase is a FIV integrase.

4. The complex of claim 3 , wherein the FIV integrase comprises an amino acid modification of H14N, E170A, or both H14N and E170A.

5. The complex of claim 1 , wherein the peptide binding domain is a WW binding domain.

6. The complex of claim 1 , wherein the exposed unstructed loop corresponds to amino acids encoded by a viral central-polypurine tract region (cPPT).

7. The complex of claim 1 , wherein the non-native protein binding site comprises a PY motif or a PGR motif.

8. The complex of claim 1 , wherein the non-native protein binding site is at least 20 amino acid in length.

9. The complex of claim 1 , wherein the non-native protein binding site is at least 15 amino acids in length.

10. The complex of claim 9 , wherein the non-native protein binding site is at least 12 amino acids in length.

11. The complex of claim 10 , wherein the non-native protein binding site is at least 10 amino acids in length.

12. The complex of claim 1 , wherein the DNA binding domain is a designed zinc finger comprising at least 2 finger modules.

13. The complex of claim 12 , wherein the zinc-finger domain is Zif268.