Image acquisition, processing, and display
Image data is acquired, processed, and/or displayed in accordance with an embodiment of the present disclosure to display, monitor, and/or demonstrate the progress of an experiment substantially in real-time and with high sensitivity. In one embodiment, at least one time-resolved value of spatially distributed polarization change data is provided and displayed. Advantageously, real-time processing and display of data is provided such that discussion and collaboration about the experiment may occur, time-resolved data is not lost, and resources are not wasted.
1. An apparatus for imaging, comprising:
a light source emitting a polarized light beam;
an optical assembly including a light reflection surface, wherein the light beam from the light source is reflected by the light reflection surface to provide an evanescent field adjacent the light reflection surface, the light reflection surface being adapted to allow placing thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam;
a two-dimensional array detector positioned to detect the spatially distributed polarization changes caused by the specimen array; and
a processor operably coupled to the two-dimensional array detector, the processor processing data from the two-dimensional array detector to provide a two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array in real-time.
2. The apparatus as in claim 1 , wherein the specimen array comprises a two-dimensional array formed of multiple fields comprising biomolecular substances.
3. The apparatus as in claim 2 , wherein the biomolecular substances are proteins, peptides, and/or polynucleotide sequences.
4. The apparatus as in claim 1 , wherein the processor calculates an intensity value of a specimen array spot over time.
5. The apparatus as in claim 4 , wherein the processor associates a color value to the calculated intensity value.
6. The apparatus as in claim 1 , wherein the processor includes a display device for displaying the two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array.
7. The apparatus as in claim 1 , wherein the processor includes a browser application for uploading the two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array to a network.
8. The apparatus as in claim 1 , wherein the processor calculates an intensity value differential of a specimen spot in the specimen array.
9. A method of imaging, comprising:
passing a polarized light beam into an optical assembly including a control layer and a light reflection surface to provide an evanescent field with controlled height and intensity adjacent the light reflection surface, a specimen array in the evanescent field causing spatially distributed polarization changes in the cross-section of the light beam;
passing the reflected light beam out of the optical structure;
detecting the spatially distributed polarization changes caused by the specimen array; and
processing the detected spatially distributed polarization changes to provide a two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array in real-time.
10. The method of claim 9 , wherein the specimen array comprises a plurality of discrete specimen spots and the image is provided for each of the discrete specimen spots.
11. The method of claim 9 , further comprising using the spatially distributed polarization changes to determine two-dimensionally distributed presence and/or properties of the specimen array constituents.
12. The method of claim 9 , wherein the specimen array is in a micro-titer plate.
13. The method of claim 12 , further comprising:
resolving the spatially distributed polarization changes for matching positions in the micro-titer plate; and
analyzing the polarization changes to determine desired characteristics in each position.
14. The method of claim 9 , further comprising analyzing the polarization changes to determine the binding characteristics of each discrete specimen spot.
15. The apparatus of claim 1 , wherein the processor is adapted to calculate at least one time-resolved value of the data from the detector including a thickness value of a specimen spot in the specimen array.