Method and apparatus for detecting conception in animals
The present invention provides antibodies which specifically bind early conception factor, which can be found in body fluids of animals including but not limited to the cow, cat, dog, horse, human, sheep, and pig. The invention provides methods for detecting conception or the absence of conception in an animal, the latter being recognized by the absence of early conception factor in a suitable body fluid collected from the animal. Apparati for detecting early conception factor in a body fluid from an animal comprising the antibodies which specifically bind early conception factor are also provided.
1. An apparatus for detecting early conception factor in a fluid sample from a subject, wherein early conception factor has a molecular weight from about 190,000 daltons to about 205,000 daltons, comprising:
a. a body portion; and
b. a support, having thereon an antibody to said early conception factor, in contact with the body portion.
2. The apparatus of claim 1 , wherein the support comprises a material that wicks a fluid.
3. The apparatus of claim 1 , wherein the antibody is conjugated to a detectable moiety.
4. The apparatus of claim 1 , wherein the support has both a monoclonal and a polyclonal antibody thereon.
5. The apparatus of claim 4 , wherein the monoclonal and polyclonal antibodies are spatially separated on the support.
6. The apparatus of claim 4 , wherein the polyclonal antibody is localized in a band, wherein the band is substantially perpendicular to the longitudinal axis of the support.
7. The apparatus of claim 4 , further comprising a means on the body portion for directing a fluid to the support.
8. The apparatus of claim 7 , wherein the means on the body portion for directing a fluid to the support, directs the fluid to a location on the support whereon the monoclonal antibody is located, whereby the sample contacts the monoclonal antibody and the support wicks the monoclonal antibody and the fluid into contact with the band containing the polyclonal antibody.