B7-4 Antibodies and uses therefor
The invention provides isolated nucleic acids molecules, designated B7-4 nucleic acid molecules, which encode novel B7-4 polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing B7-4 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a B7-4 gene has been introduced or disrupted. The invention still further provides isolated B7-4 proteins, fusion proteins, antigenic peptides and anti-B7-4 antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
1. An isolated antibody which selectively binds to the polypeptide selected from the group consisting of:
a) the polypeptide consisting of the sequence from about amino acid residue 19 to amino acid residue 245 of SEQ ID NO:2;
b) the polypeptide consisting of the sequence from about amino acid residue 19 to amino acid residue 238 of SEQ ID NO:4; and
c) the polypeptide consisting of the sequence from about amino acid residue 19 to amino acid residue 227 of SEQ ID NO:4.
2. A method for detecting the presence of a B7-4 polypeptide in a sample comprising:
a) contacting the sample with the isolated antibody of claim 1 ; and
b) determining whether the compound binds to the polypeptide in the sample to thereby detect the presence of a B7-4 polypeptide in the sample.
3. An isolated antibody which selectively binds to the polypeptide of SEQ ID NO:2 or SEQ ID NO:4.
4. The isolated antibody of claim 1 or 3 , wherein said isolated antibody is polyclonal, monoclonal, chimeric, human, humanized, bispecific, multispecific, primatized, or an antibody fragment.
5. The isolated antibody of claim 4 , wherein said isolated antibody is a Fab, F(ab′) 2 , single chain, scFv, or Fd antibody, or fragments thereof.
6. The isolated antibody of claim 1 or 3 , wherein said isolated antibody is attached to a solid substrate.
7. The isolated antibody of claim 1 or 3 , wherein the isolated antibody upmodulates an immune response in a subject.
8. The isolated antibody of claim 1 or 3 , wherein the isolated antibody downmodulates an immune response in a subject.
9. An isolated cell that is capable of expressing the isolated antibody of claim 1 or 3 .
10. A composition, comprising the isolated antibody of claim 1 or 3 and a pharmaceutically acceptable excipient.
11. The composition of claim 10 , wherein said isolated antibody is polyclonal, monoclonal, chimeric, human, humanized, or an antibody fragment.
12. The composition of claim 11 , wherein said isolated antibody is a Fab, F(ab′) 2 , single chain, scFv, or Fd antibody, or fragments thereof.
13. The composition of claim 10 , wherein said composition upmodulates an immune response in a subject.