Methods for detecting pathogen specific nucleic acids in urine
The invention is based upon the discovery that small nucleic acids from non-viral pathogens are able to cross the kidney and are present in urine of a subject when the subject is infected with the non-viral pathogen. These transrenal DNAs are especially prevalent at smaller sizes under about 300 bp. Thus the invention provides compositions and methods for the diagnosis of infection of a subject with non-viral pathogens through the detection of transrenal nucleic acids from those pathogens in the urine of the subject.
1. A method for diagnosing a Mycobacterium tuberculosis infection in a subject, comprising:
a) obtaining a urine sample from a subject;
b) separating a soluble fraction of said urine sample; and
c) detecting the presence of a cell-free Mycobacterium tuberculosis nucleic acid in said soluble fraction of said urine sample, wherein said nucleic acid is less than about 300 bp in length, wherein said detecting is performed by a method selected from the group consisting of PCR, nested PCR, semi-nested PCR, LCR, and SDA, using a primer set comprising at least one forward primer selected from the group consisting of SEQ ID NOs: 34, 37 and 39, and at least one reverse primer selected from the group consisting of SEQ ID NOs: 35, 36, 38 and 40, wherein the presence of said nucleic acid diagnoses a Mycobacterium tuberculosis infection.
2. The method of claim 1 , further comprising the step of quantitating the nucleic acid.
3. The method of claim 1 , wherein the subject is a mammal.
4. The method of claim 3 , wherein the mammal is a human.