IP Library Granted Patent US 8,551,967
Granted Patent B2
US 8,551,967 · App. 11/366,243 · Granted Oct 8, 2013

Formulations with anti-tumour action

Inventors: Laura Iris Ferro (Milan, IT); Massimo Iacobelli (Milan, IT); Paul Richardson (Wellesley, MA)
Assignee: Gentium SpA
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Quick Facts
Patent No.
US 8,551,967
App. No.
11/366,243
Granted
Oct 8, 2013
Kind
B2
Abstract

The use of defibrotide as an anti-tumor agent, alone or in combination with other active ingredients with anti-tumor action, is described.

Claims (46)

1. A method for treating a human affected by multiple myeloma, said method consisting essentially of administering to said human an effective amount of defibrotide sufficient to treat the multiple myeloma, the defibrotide obtained by extraction from animal tissue and having CAS registry number 83712-60-1.

2. A method according to claim 1 , wherein defibrotide is administered intravenously.

3. A method according to claim 1 , wherein the defibrotide comprises an oligonucleotide and/or polynucleotide extracted from animal tissue.

4. A method according to claim 1 , wherein the defibrotide comprises a polydeoxyribonucleotide obtained by extraction from animal tissue, in the form of an alkali-metal salt.

5. A method for treating a human affected by multiple myeloma, said method consisting essentially of administering to said human an effective amount of defibrotide sufficient to treat the multiple myeloma, the defibrotide obtained by extraction from animal tissue and comprising a polydeoxyribonucleotide corresponding to the following formula of random sequence:

P 1-5 ,(dAp) 12-24 ,(dGp) 10-20 ,(dTp) 13-26 ,(dCp) 10-20 ,

Wherein

P=phosphoric radical

dAp=deoxyadenylic monomer

dGp=deoxyguanylic monomer

dTp=deoxythymidylic monomer

dCp=deoxycytidylic monomer,

with the following chemico-physical properties:

electrophoresis=homogeneous anodic mobility;

extinction coefficient, E 1cm 1% at 260±1 nm=220±10°;

extinction reaction, E 230 /E 260 =0.45±0.04;

coefficient of molar extinction (referred to phosphorus),

ε (P)=7.750±500;

rotary power [α] D 20° =53°±6;

reversible hyperchromicity, indicated as % in native DNA, h=15±5; and

a purine:pyrimidine ratio of 0.95±0.5.

6. A method according to claim 5 , wherein defibrotide is administered intravenously.

7. A method according to claim 5 wherein the defibrotide comprises an oligonucleotide and/or polynucleotide.

8. A method according to claim 5 wherein the defibrotide comprises a polydeoxyribonucleotide obtained by extraction from animal tissue, in the form of an alkali-metal salt.

9. A method according to claim 1 wherein the method consists of administering to said human an effective amount of defibrotide sufficient to treat the multiple myeloma, the defibrotide obtained by extraction from animal tissue and having CAS registry number 83712-60-1.

10. A method for treating a human affected by multiple myeloma, said method comprising administering to said human an effective amount of defibrotide sufficient to treat the multiple myeloma, the defibrotide obtained by extraction from animal tissue and having CAS registry number 83712-60-1.

11. A method according to claim 10 , wherein defibrotide is administered intravenously.

12. A method according to claim 10 wherein the defibrotide comprises an oligonucleotide and/or polynucleotide extracted from animal tissue.

13. A method according to claim 10 wherein the defibrotide comprises a polydeoxyribonucleotide obtained by extraction from animal tissue, in the form of an alkali-metal salt.

14. A method according to claim 10 wherein the defibrotide comprises a polydeoxyribonucleotide corresponding to the following formula of random sequence:

P 1-5 ,(dAp) 12-24 ,(dGp) 10-20 ,(dTp) 13-26 ,(dCp) 10-20 ,

Wherein

P=phosphoric radical

dAp=deoxyadenylic monomer

dGp=deoxyguanylic monomer

dTp=deoxythymidylic monomer

dCp=deoxycytidylic monomer,

with the following chemico-physical properties:

electrophoresis=homogeneous anodic mobility;

extinction coefficient, E 1cm 1% at 260±1 nm=220±10°;

extinction reaction, E 230 /E 260 =0.45±0.04;

coefficient of molar extinction (referred to phosphorus),

ε (P)=7.750±500;

rotary power [ α ]D 20° 53°±6;

reversible hyperchromicity, indicated as % in native DNA, h=15±5; and

a purine:pyrimidine ratio of 0.95±0.5.

Assignments (2)
CHANGE OF NAME Recorded Oct 11, 2016
From: GENTIUM S.P.A.
To: GENTIUM S.R.L.
Reel/Frame 040314/0568 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 26, 2006
From: FERRO, LAURA IRIS; IACOBELLI, MASSIMO; RICHARDSON, PAUL
To: GENTIUM SPA
Reel/Frame 017916/0326 →
Priority Claims (1)
IT MI2003A001714 · Sep 5, 2003 · national
Continuity (3)
Continuation In Part PCTEP2004009723 · Aug 27, 2004
Provisional Application 60539344 · Jan 28, 2004
Related Publication 20060211646A1 · Sep 21, 2006