Oil-in-water method for making polymeric implants containing a hypotensive lipid
Biocompatible microparticles include an ophthalmically active cyclic lipid component and a biodegradable polymer that is effective, when placed into the subconjunctival space, in facilitating release of the cyclic lipid component into the anterior and posterior segments of an eye for an extended period of time. The cyclic lipid component can be associated with a biodegradable polymer matrix, such as a matrix of a two biodegradable polymers. Or, the cyclic lipid component can be encapsulated by the polymeric component. The present microparticles include oil-in-water emulsified microparticles. The subconjunctivally administered microparticles can be used to treat or to reduce at least one symptom of an ocular condition, such as glaucoma or age related macular degeneration.
1. An oil-in-water emulsion method for making prostamide-encapsulated microspheres, the method comprising:
(a) combining a poly(lactide-co-glycolide) (PLGA) copolymer with a prostamide in an organic solvent to form an oil phase;
(b) combining polyvinyl alcohol (PVA) with an excess amount of said prostamide in water to form a prostamide-saturated aqueous phase;
(c) emulsifying the oil phase with the prostamide-saturated aqueous phase by mixing the two phases together to thereby form prostamide-encapsulated microspheres; and
(d) removing the organic solvent from the microspheres.
2. The method of claim 1 , wherein the PLGA copolymer has a lactide/glycolide ratio of 75/25, a molecular weight of about 63 kilodaltons, and an inherent viscosity of about 0.6 dL/g.
3. The method of claim 1 , wherein the organic solvent is dichloromethane.
4. The method of claim 1 , wherein the prostamide is bimatoprost.
5. The method of claim 1 , wherein removing the organic solvent from the microspheres comprises evaporation of the organic solvent.
6. The method of claim 1 , further comprising terminally sterilizing the prostamide-encapsulated microspheres with gamma radiation.
7. The method of claim 1 , wherein the prostamide is bimatoprost and wherein solid bimatoprost is present in the emulsion during preparation such that the bimatoprost exceeds its solubilities in both the aqueous and the oil phases.
8. The method of claim 1 , wherein the prostamide is bimatoprost, the organic solvent is dichloromethane, and wherein the oil phase is emulsified with the aqueous phase by drop wise addition of the oil phase into the aqueous phase under continuous mixing with a high shear impeller.
9. The method of claim 1 , wherein the organic solvent is removed from the microspheres by extraction of the solvent in a continuous flow of purified water.
10. The method of claim 1 , wherein the prostamide is bimatoprost and the organic solvent is ethyl acetate or methylene chloride.