Antimicrobial composition and method for making same
Anti-microbial Formulations and methods of their use and production are disclosed. The Formulations of the present invention are effective as broad spectrum anti-bacterial agents with efficacy against both Gram-negative and Gram-positive bacteria, as anti-viral agents with efficacy against both enveloped and non-enveloped viruses, anti fungal agents and anti spore forming agents. The present invention includes anti-microbial Formulations that include at least one surfactant, optionally at least one acid, at least one non-cationic anti-microbial agent, and optionally water. The anti-microbial Formulations of the present invention may additionally contain an organic salt. The organic salt may be a salt of the same acid that is used in the Formulation or a salt of a different acid.
1 . An anti-microbial composition comprising:
at least one surfactant present in a concentration from about 3% to about 95% by weight;
an acid present in a concentration of up to about 20% by weight;
at least one non-cationic anti-microbial agent and the balance water,
wherein the amount of anti-microbial agent is effective to produce a Zone of Inhibition of from 15 to 150 mm and for the composition to exhibit a Plate Cidality of no growth against each of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027 and Candida albicans ATCC 10231 at aqueous dilutions up to 1:2000 eliminate”) and is CPE negative.
2 . The antimicrobial composition of claim 1 wherein the Zone of Inhibition is in the range of from about 30 to 110.
3 . The antimicrobial composition of claim 2 wherein the Zone of Inhibition is in the range of from about 45 to 75.
4 . The antimicrobial composition of claim 1 which is effective to produce a Zone of Inhibition against Staphylococcus aureus ATCC 6538 of from 50 to 65.
5 . The antimicrobial composition of claim 1 which is effective to produce a Zone of Inhibition against Escherichia coli ATCC 8739 of from 50 to 65.
6 . The antimicrobial composition of claim 1 which is effective to produce a Zone of Inhibition against Bacillus subtilus and Bacillus anthracis of from 65 to 90 and a Plate Cidality of no growth against Bacillus subtilus and Bacillus anthracis.
7 . The anti-microbial Formulation of claim 1 , wherein said surfactant is selected from the group consisting of amphoteric surfactant, cationic surfactant, nonionic surfactant, anionic surfactant, and combinations thereof.
8 . The anti-microbial composition of claim 1 , wherein said compositions includes a first surfactant and a second surfactant.
9 . The anti-microbial Formulation of claim 8 , wherein said first surfactant is an amphoteric surfactant and said second surfactant is an anionic surfactant.
10 . The anti-microbial composition of claim 1 , wherein said surfactant is selected from the group consisting of alkylamine oxides, alkyamidopropyl amine oxides, alkyl betaines, alkyamidopropyl betaines, and sultaines.
11 . The anti-microbial composition of claim 10 , wherein said alkylamine oxide is selected from the group consisting of octyl amine oxide, decyl amine oxide, lauryl amine oxide, iso-dodecyl amine oxide, myristyl amine oxide, cetyl amine oxide, oleamine oxide, stearyl amine oxide, and palmitamine oxide.
12 . The anti-microbial composition of claim 10 , wherein said alkylamidopropyl amine oxide is selected from the group consisting of laurylamidopropyl amine oxide, cocamidopropyl amine oxide, stearamidopropyl amine oxide, germamidopropyl amine oxide.
13 . The anti-microbial composition of claim 10 , wherein said alkyl betaine is selected from the group consisting of octyl betaine, lauryl betaine, cocobetaine, cetyl betaine, oleyl betaine, and tallow dihydroxylethyl glycinate.
14 . The anti-microbial composition of claim 10 , wherein said alkylamidopropyl betaine is selected from the group consisting of caprylamidopropyl betaine, capramidopropyl betaine, lauamidopropyl betaine, cocamidopropyl betaine, isostearamidopropyl betaine, wheatgermaidopropyl betaine, and coco/sunfloweramidopropyl betaine.
15 . The anti-microbial composition of claim 10 , wherein said sultaine is selected from the group consisting of cocamidopropyl hydroxysultaine and lauryl hydroxysultaine.
16 . The anti-microbial composition of claim 1 , wherein said surfactant is selected from the group consisting of alkali metal salts of alkylamphoacetates, dialkali metal salts of alkylamphoacetates, alkali metal salts of alkylamphopropionates, dialkali metal salts of alkylamphopropionates, alkanolamides, disodium alkyl sulfosuccinates, monsodium diakyl sulfosuccinates, disodium alkylethoxy sulfosuccinates, sodium ethoxydimethicone sulfosuccinates, disodium propoxydimethicone sulfosuccinates, disodium alkylamido MEA sulfosuccinates, disodium alkylamido MPA sulfosuccinates, sodium alkylamidopropoxy sulfosuccinates, alkylamidopropylmorpholine lactate, and ethylene oxie/propylene oxide diblock and triblock surfactants.
17 . The anti-microbial composition of claim 1 , wherein said acid is an organic acid or an inorganic acid.
18 . The anti-microbial composition of claim 17 , wherein said organic acid is citric acid.
19 . The anti-microbial composition of claim 17 , wherein said inorganic acid is phosphoric acid.
20 . The anti-microbial composition of claim 1 , wherein said salt is an organic salt or an inorganic salt.
21 . The anti-microbial composition of claim 20 , wherein said organic salt is a citrate.
22 . The anti-microbial composition of claim 20 , wherein said inorganic salt is ammonium bifluoride.
23 . The anti-microbial composition of claim 1 , wherein said non-cationic antimicrobial agent is selected from the group consisting of phenolics, halogenated phenolics, hogenated diphenyl ethers, halogenated carbonilides, water soluble or water insoluble peroxy oxidizing agents, and combinations thereof.
24 . The anti-microbial composition of claim 23 , wherein said peroxy oxidizing agent is selected from the group consisting of peroxides, peresters, peracids, and persulfates.
25 . The anti-microbial composition of claim 24 , wherein said peroxy oxidizing agent is the group consisting of hydrogen peroxide, t-butyl hydroperoxide, cumene hydroperoxide and peracetic acid.
26 . The anti-microbial composition of claim 1 , wherein said non-cationic anti-microbial agent is Triclosan.
27 . The anti-microbial composition of claim 7 , wherein said amphoteric surfactant is cocobetaine.
28 . The anti-microbial composition of claim 7 , wherein said surfactant is an alkyl amine oxide.
29 . The anti-microbial composition of claim 28 , wherein said surfactant is lauryl amine oxide.
30 . The antimicrobial composition of claim 7 wherein said anionic surfactant is a sulfosuccinate.
31 . The anti-microbial composition of claim 1 , wherein said anti-microbial composition possesses broad spectrum anti-microbial activity.
32 . The anti-microbial composition of claim 31 , wherein said broad spectrum anti-microbial activity includes anti-bacterial activity against Gram-negative bacteria and Gram-positive bacteria.
33 . The anti-microbial composition of claim 1 , wherein said anti-microbial composition is a clear and homogeneous composition.
34 . A method of formulating an anti-microbial composition, comprising the steps of:
forming an admixture of water, and at least one surfactant;
heating said admixture to the melting point of a first solid non-cationic anti-microbial agent;
adding the non-cationic anti-microbial agent to said admixture while rapidly stirring at this melting point temperature; and
cooling said admixture to approximately room temperature,
35 . The method of claim 34 wherein at least one acid is added to the admixture of water and surfactant.
36 . The method of claim 34 wherein a second noncationic antimicrobial is added at room temperature after said cooling step.
37 . The method of claim 34 , further comprising the step of adding a peroxy oxidizing agent to said admixture after said cooling step.
38 . A method of inhibiting the growth of microbes on a surface comprising the step of applying the composition of claim 1 to a surface.
39 . A method of inhibiting the growth of microbes in a liquid, comprising the step of mixing the composition of claim 1 into a liquid in which microbial growth is desired to be inhibited.
40 . A method of inhibiting the growth of microbes on the skin comprising applying the composition of claim 1 in combination with water to the skin.
41 . A method of formulating an anti-microbial composition composed of:
at least one surfactant present in a concentration from about 5% to about 95% by weight;
at least one non-cationic anti-microbial agent present in a concentration of up to about 20% by weight; and
the balance water; comprising the steps of:
forming an admixture of said water, and said at least one surfactant;
heating said admixture to the melting point of a first non-cationic anti-microbial agent;
adding said non-cationic anti-microbial agent to said admixture while rapidly stirring at this melting point temperatures; and
cooling said admixture to approximately room temperature.
42 . The method of claim 41 which further comprises adding at least one acid to the admixture in a concentration of up to about 20% by weight.
43 . A method of formulating an anti-microbial composition composed of:
at least one surfactant present in a concentration from about 5% to about 95% by weight;
at least one non-cationic anti-microbial agent present in a concentration of up to about 20% by weight; and
the balance water; comprising the steps of:
mixing a solid antimicrobial agent with a liquid antimicrobial agent capable of dissolving or liquefying the solid antimicrobial agent, at ambient temperatures and agitating the mixture until a clear homogeneous Formulation is produced.
44 . The method of claim 43 which further comprises adding at least one acid to the admixture in a concentration of up to about 20% by weight.
45 . The method of claim 44 in which an aqueous solution of a peroxide is the liquid antimicrobial agent.
46 . An antimicrobial composition obtained by the method of claim 43 .
47 . A method for killing microbes on a surface comprising applying antimicrobial cidal effective amount of the composition of claim 1 to the surface.
48 . A method for rendering the atmosphere of an enclosed space antimicrobial comprising applying an antimicrobial effective amount of the composition of claim 1 to the atmosphere in the enclosed space.
49 . An antimicrobial composition obtained by the method of claim 45 .
50 . An antimicrobial formulation comprising about 6.67 wt. % cocobetaine—low salt version, about 0.13 wt. % lauryl amine oxide, about 0.13 wt. % citric acid, about 0.73 wt, % triclosan, about 3.00 wt. % t-butyl hydroperoxide and about 13 ppm iron sulfate, the balance water.
51 . An antimicrobial formulation comprising about 4% cocobetaine-low salt version, about 0.13% lauryl amine oxide, about 0.73% Triclosan, about 3% t-butyl hydroperoxide, about 2% peracetic acid and about 2% stabilized hydrogen peroxide, the balance water.
52 . A method for preparing a stable peroxy antimicrobial formulation comprising adding from about 5 to 70% by weight of stabilized hydrogen peroxide solution to a surfactant solution, heating the mixture at approximately 60° C. for about 8 to 12 minutes, adding a noncationic antimicrobial agent with rapid stirring to obtain a clear homogenous low viscosity solution, stirring the solution at about 60-63° C. for 10 minutes, cooling the formulation to room temperature, adding an anti-microbial peroxide component and stirring the mixture for one to five minutes to form a clear and homogeneous solution which does not degas.
53 . The method of claim 52 wherein the amount of stabilized hydrogen peroxide added is between 15 and 50% by weight of stabilized hydrogen peroxide.
54 . The method of claim 52 wherein the resulting formulation is diluted with a solution of stabilized hydrogen peroxide solution, an aqueous solution of iron sulfate or water.