Capillary array and capillary electrophoresis apparatus
View Patent ↗In a case where the temperature of capillaries cannot be controlled rapidly in accordance with the change of the amount of heat generated from the capillaries, the phoretic speed of the sample is not controlled constant to lower the analyzing performance for the sample. This invention intends to stabilize the electrophoretic speed. The surface of a substrate on which a plurality of capillaries are arranged is in contact with a member capable of temperature control thereby controlling the temperature of the capillaries. Since a member capable of controlling the temperature and the capillaries are in direct contact with each other according to the invention, the temperature of the capillary can be controlled rapidly at a high accuracy, so that electrophoretic speed is stabilized.
1. An electrophoresis apparatus including:
a plurality of capillaries that can be filled with a phoretic medium;
a support plate having the plurality of capillaries arranged on the surface thereof;
a heater in direct contact with the plurality of capillaries and the surface of a support plate to directly control the temperature of the plurality of capillaries;
a power supply that applies a voltage on both ends of a capillary; and
an optical system that irradiates an excitation light to an electrophoretically separated sample and detecting fluorescence from the sample.
2. An electrophoresis apparatus according to claim 1 , wherein
the support plate supports a detection base plate in which the plurality of capillaries irradiated with the excitation light are arranged and disposed.
3. An electrophoresis apparatus according to claim 1 , wherein
the support plate has an electrode holding member holding a plurality of electrodes corresponding to the plurality of capillaries.
4. An electrophoresis apparatus according to claim 1 , further including a phoretic medium filling mechanism that fills a phoretic medium from a phoretic medium injection end of the plurality of capillaries, wherein the support base supports a capillary head that bundles the phoretic medium injection ends of the plurality of capillaries and can be connected with the phoretic medium filling mechanism.
5. An electrophoresis apparatus according to claim 4 , wherein
a plurality of phoretic medium injection ends are held being protruded from the capillary heads, and
the plural phoretic medium injection ends are directed downward when the capillary head is connected with the phoretic medium filling mechanism.
6. An electrophoresis apparatus according to claim 4 , wherein
the support plate movably supports the capillary heads.
7. An electrophoresis apparatus according to claim 1 , wherein
the support base has a light shielding member covering the plurality of capillaries arranged in parallel and has plural detection windows for transmitting fluorescence from samples, and
plural detection windows are not arranged in the direction substantially perpendicular to the capillaries.
8. An electrophoresis apparatus according to claim 7 , wherein
the detection windows are arranged stepwise.
9. An electrophoresis apparatus according to claim 1 , wherein
a light permeable region capable of transmitting a light is provided on the portion of the support base,
a portion of the plurality of capillaries is disposed on a light transmission region, and
an excitation light is irradiated to the capillaries on the side opposite to the surface of the support plate on which the capillaries are fixed.
10. An electrophoresis apparatus according to claim 1 , wherein
a portion of the capillary is fixed to the support base plate and the plurality of capillaries are arranged as a capillary array.
11. An electrophoresis apparatus according to claim 1 , wherein
the plurality of capillaries arranged on the support plate do not intersect with each other.