Ghrelin binding nucleic acids
The present invention is related to a nucleic acid, preferably binding to ghrelin, whereby the nucleic acid comprises a first stretch Box A, and a second stretch Box B, whereby the first stretch Box A comprises about 25 consecutive nucleotides, the second stretch Box B comprises about six to eight consecutive nucleotides, whereby a 3′-terminal stretch of nucleotides of the first stretch Box A hybridizes with the second stretch Box B, whereby upon hybridization a first double-stranded structure is formed, whereby such first double-stranded structure comprises a bulge.
1. An isolated nucleic acid having the nucleotide sequence set forth in SEQ ID NO: 39 wherein said isolated nucleic acid binds a ghrelin.
2. A kit for the detection of ghrelin, comprising the nucleic acid according to claim 1 .
3. The nucleic acid of claim 1 , wherein said nucleic acid further comprises a hydroxyethyl starch (HES) moiety, a polyethylene glycol (PEG) moiety or both.
4. The nucleic acid of claim 3 , wherein the molecular weight of said PEG moiety is from 20 to 120 kD.
5. The nucleic acid of claim 3 , wherein the molecular weight of said PEG moiety is from 30 to 80 kD.
6. The nucleic acid of claim 3 , wherein the molecular weight of said PEG moiety is 40 kD.
7. The nucleic acid of claim 3 , wherein the HES moiety has a molecular weight from 10 to 130 kD.
8. The nucleic acid of claim 3 , wherein said HES moiety has a molecular weight from 30 to 80 kD.
9. The nucleic acid of claim 3 , wherein said HES moiety has a molecular weight of 50 kD.
10. The nucleic acid of claim 1 , wherein one or more nucleotides of said nucleic acid are L nucleotides.
11. The nucleic acid of claim 1 , wherein all of the nucleotides of said nucleic acid are L nucleotides.