Δ 4,5 glycuronidase nucleic acid compositions
The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof.
1. An isolated nucleic acid molecule selected from the group consisting of
(a) nucleic acid molecules which hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence set forth as SEQ ID NO:2, wherein the hybridization conditions are hybridization at 65° C. in hybridization buffer comprising 3.5×(0.15M sodium chloride/0.015M sodium citrate, pH7), 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% bovine serum albumin, 25 mM NaH 2 PO 4 , pH 7, 0.5% sodium dodecylsulphate and 2 mM ethylene diamine tetra acetic acid,
(b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence only due to degeneracy of the genetic code, and
(c) full complements of (a) or (b).
2. The isolated nucleic acid molecule of claim 1 , wherein the isolated nucleic acid molecule codes for SEQ ID NO:1.
3. The isolated nucleic acid molecule of claim 1 , wherein the isolated nucleic acid molecule comprises the nucleotide sequence set forth as SEQ ID NO:2.
4. An expression vector comprising the isolated nucleic acid molecule of any of claims 1 - 3 operably linked to a promoter.
5. A host cell comprising the expression vector of claim 4 .
6. A composition comprising the nucleic acid of any one of claims 1 - 3 and a pharmaceutically acceptable carrier.
7. A composition comprising:
the vector of claim 4 and a pharmaceutically acceptable carrier.
8. A composition comprising:
the host cell of claim 5 and a pharmaceutically acceptable carrier.