Methods and apparatus for amplification of DNA using sonic energy
Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
1. A method for amplification of DNA, comprising the steps of:
providing a reaction mixture cocktail including DNA to be amplified, a polymerase enzyme, nucleosides, and primers;
providing a sonic energy source suitable for dissociating said DNA to be amplified from its double stranded form to single strands of complementary DNA;
operating the sonic energy source in order to effect dissociation of double stranded DNA in the mixture into single strands of DNA without raising the temperature of said reaction mixture cocktail to a temperature which would effect thermal denaturation of the double stranded DNA;
discontinuing operation of the sonic energy source in order to allow formation of double stranded DNA from the single strands of DNA without including a cooling step in order to allow formation of double stranded DNA;
repeating operation of the sonic energy source and discontinuance of operation of the sonic energy source as many times as required to produce a desired amount of DNA.
2. The method of claim 1 further comprising the step of monitoring the status of amplification over time.
3. The method of claim 2 further comprising the step of operating the sonic energy source until the monitoring step indicates that dissociation is complete.
4. The method of claim 2 further comprising the step of discontinuing operation of the sonic energy source until the formation of double stranded DNA from single strands of DNA is complete.
5. The method of claim 1 , wherein the step of operating the sonic energy source lasts two seconds, and the step of discontinuing operation of the sonic energy source lasts for 11 seconds.
6. The method of claim 1 , wherein the sonic energy is at least 20 KHz.
7. The method of claim 1 , wherein the sonic energy produced by the sonic energy source is at or near the resonance frequency of said sonic energy source.
8. The method of claim 1 , further comprising the step of monitoring the observable presence of DNA amplification in order to provide a quantitative determination of the presence of a target DNA in a sample.
9. The method of claim 1 , further comprising the step of raising the temperature of the sample above ambient temperature in order to improve efficiency of PCR.
10. A method for amplification of DNA, comprising the steps of:
providing a reaction mixture cocktail including DNA to be amplified, a polymerase enzyme, nucleosides, and primers;
providing a sonic energy source suitable for dissociating said DNA to be amplified from its double stranded form to single strands of complementary DNA;
operating the sonic energy source in order to effect dissociation of double stranded DNA in the mixture into single strands of DNA;
discontinuing operation of the sonic energy source in order to allow formation of double stranded DNA from the single strands of DNA;
repeating operation of the sonic energy source and discontinuance of operation of the sonic energy source as many times as required to produce a desired amount of DNA,
wherein said steps of operating the sonic energy source and discontinuing operation of the sonic energy source effect amplification of DNA without thermal cycling.
11. The method of claim 10 further comprising the step of monitoring the status of amplification over time.
12. The method of claim 11 further comprising the step of operating the sonic energy source until the monitoring step indicates that dissociation is complete.
13. The method of claim 12 further comprising the step of discontinuing operation of the sonic energy source until the formation of double stranded DNA from single strands of DNA is complete.
14. The method of claim 10 , wherein the step of operating the sonic energy source lasts two seconds, and the step of discontinuing operation of the sonic energy source lasts for 11 seconds.
15. The method of claim 10 , wherein the sonic energy is at least 20 KHz.
16. The method of claim 10 , wherein the sonic energy produced by the sonic energy source is at or near the resonance frequency of said sonic energy source.
17. The method of claim 10 , further comprising the step of monitoring the observable presence of DNA amplification in order to provide a quantitative determination of the presence of a target DNA in a sample.
18. The method of claim 10 , further comprising the step of raising the temperature of the sample above ambient temperature in order to improve efficiency of PCR.