Aggregometer with near ultraviolet light source
An aggregometer has a radiation path along which radiation can pass, a sample receiving area located along the radiation path, a short-wavelength LED that transmits radiation along the radiation path through the sample receiving area, a detector positioned along the radiation path for detecting radiation transmitted from the short-wavelength LED, and a processor that controls intensity of radiation transmitted from the short-wavelength LED.
1. A method for testing a fluid comprising:
providing a plasma sample including an un-reacted reagent;
transmitting a beam of short-wavelength radiation through the plasma sample at a predetermined transmission level;
detecting an attenuation of the beam of radiation at the predetermined transmission level through the un-reacted plasma sample;
detecting an attenuation of the beam of radiation at the predetermined transmission level through the plasma sample during a period of reaction of the reagent in the plasma sample; and
comparing the attenuation of the beam of radiation through the plasma sample during the reaction period with the beam of radiation during the period of non-reaction.
2. A method for testing a fluid comprising the steps of:
providing a sample including an un-reacted reagent;
transmitting a beam of short-wavelength radiation through the sample at an original transmission intensity;
detecting an attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the un-reacted reagent;
increasing the transmission intensity of the beam of short-wavelength radiation until the attenuated intensity corresponds to the original transmission intensity;
allowing the un-reacted reagent to react; and
detecting a subsequent attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation at the predetermined transmission level passes through the reagent during a time period while the reagent is reacting.
3. The method of claim 1 , wherein the increased transmission intensity of the beam of short-wavelength radiation is set to a level such that radiation is detectable through the reagent during the time period.
4. The method of claim 2 , wherein the short-wavelength radiation is transmitted in a wavelength range between 275 and 500 nm.
5. The method of claim 4 , wherein the short-wavelength radiation is transmitted in a wavelength range between 350 and 450 nm.
6. The method of claim 5 , wherein the short-wavelength radiation is transmitted in a wavelength range between 390 and 410 nm.
7. A method for testing a fluid comprising the steps of:
providing a substantially platelet-free plasma sample;
transmitting a beam of short-wavelength radiation through the substantially platelet-free plasma sample at an original transmission intensity;
detecting an attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the substantially platelet-free plasma sample;
providing a plasma sample with substantially unaggregated platelets;
transmitting a beam of short-wavelength radiation through the sample with substantially unaggregated platelets at the original transmission intensity;
detecting an attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample with substantially unaggregated platelets; and
detecting an attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample during a period of aggregation of the platelets.
8. The method of claim 7 , further comprising the step of:
comparing (1) the detected attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the substantially platelet-free plasma sample with (A) the detected attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample with substantially unaggregated platelets and/or
(B) the detected attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample during a period of aggregation of the platelets.
9. The method of claim 8 , further comprising the step of:
outputting a comparison between (1) the intensity of the beam of short-wavelength radiation passing through the substantially platelet-free plasma sample at the original transmission intensity, with (A) the detected attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample with substantially unaggregated platelets and/or
(B) the detected attenuated intensity of the beam of short-wavelength radiation after the beam of short-wavelength radiation passes through the plasma sample during a period of aggregation of the platelets.
10. The method of claim 7 , wherein the short-wavelength radiation is transmitted in a wavelength range between 275 and 500 nm.
11. The method of claim 7 , wherein the short-wavelength radiation is transmitted in a wavelength range between 350 and 450 nm.
12. The method of claim 7 , wherein the short-wavelength radiation is transmitted in a wavelength range between 390 and 410 nm.