Culture of goblet cells
View Patent ↗The invention encompasses isolation, culture and characterization of goblet cells in vitro from mammalian conjuctiva. Goblet cells can be cultured from conjunctiva of such mammals as, e.g., humans, rats, mice, rabbits and the like. In another aspect of the invention, the culture of goblet cells has a concentration of pure goblet cells of 10% or greater. In a further embodiment, the invention comprises an immortalized goblet cell line.
1. A mammalian goblet cell line that is immortalized, wherein goblet cells for said line are derived from a method of producing a culture of goblet cells comprising the steps of:
(a) providing an explant of conjunctival mammalian tissue;
(b) culturing said explant in a growth medium;
(c) allowing said explant to grow until cell growth in the form of nodules is observed around said explant;
(d) removing said explant, leaving said nodules in said growth medium;
(e) allowing cells from said nodules to grow to form said culture of goblet cells; and
(f) genetically engineering cells from said culture of goblet cells to form a culture of immortalized said goblet cells.
2. The goblet cell line of claim 1 , wherein, in said method for deriving said cell line, said conjunctival mammalian tissue is from a human mammal.
3. The goblet cell line of claim 1 , wherein, in said method for deriving said cell line, said conjunctival mammalian tissue comprises the fornical region.
4. The goblet cell line of claim 1 , wherein, in said method for deriving said cell line, said conjunctival mammalian tissue comprises the nictitating membrane.
5. The goblet cell line of claim 1 , wherein, in said method for deriving said cell line, said conjunctival mammalian tissue is from a mammal selected from the group consisting of human, rat, mouse, rabbit, cat, dog, sheep, goat, cow, and pig.
6. A kit for examining mucin from goblet cells comprising goblet cells according to claim 1 and instructions for use thereof.