Cell-containing sheet

1. A method for producing a cell-containing fine pattern sheet comprising cells and a support comprising a bioabsorbable material that has a cell adhesion protein-containing layer on the surface thereof, comprising the steps of:

a) providing epithelial cells, epidermal cells, renal cells, muscle cells, neural cells, hepatocytes, Langerhans cells, osteocytes, chondrocytes, lymphangial cells, or periodontal ligament-derived cells;

b) contacting said cells with the surface of a substrate for cell arrangement having a cell adhesiveness-variation pattern comprising cell adhesiveness promoted regions having a water contact angle from 10° to 40°, and cell adhesiveness inhibited regions, wherein said cells adhere solely to the cell adhesiveness promoted regions of said substrate;

c) culturing said cells adhering to the surface of said substrate;

d) contacting said substrate adherent cells with a cell adhesion protein-containing layer on the surface of a support wherein said substrate adherent cells also adhere to said support, wherein the cell adhesion protein-containing layer has not been subjected to a patterning treatment; and

e) removing the substrate for cell arrangement to transfer said substrate adherent cells to said support;

wherein the support comprises an amnion in which a layer composed of cells is removed, and the support is supported by a biodegradable polymer, wherein the amnion consists of stratum compactum and basal lamina,

and wherein the cell-containing fine pattern sheet has a pattern not more than 3 mm in size and is an implant.

2. The method according to claim 1 , wherein the support further comprises a biodegradable polymer material and wherein the amnion is supported by the material.

3. The method according to claim 1 , wherein the cell adhesiveness-variation pattern is formed with a cell adhesiveness-variable layer comprising a cell adhesiveness-variable material that experiences a change in cell adhesiveness due to photocatalytic action upon energy irradiation.

4. The method according to claim 3 , wherein the cell adhesiveness-variable layer is a photocatalyst-containing cell adhesiveness-variable layer comprising a photocatalyst and a cell adhesiveness-variable material.

5. The method according to claim 3 , wherein the cell adhesiveness-variable layer has a photocatalyst treatment layer containing photocatalyst and a cell adhesiveness-variable material layer containing a cell adhesiveness-variable material, which is formed on the photocatalyst treatment layer.

6. The method according to claim 5 , wherein the cell adhesiveness-variation pattern is formed via energy irradiation after placing the cell adhesiveness-variable layer that contains a cell adhesiveness-variable material and a photocatalyst-containing layer that contains a photocatalyst in a manner such that both layers are opposed to each other.

7. The method according to claim 1 , wherein the cells with adhesiveness to a substrate for cell arrangement are human periodontal ligament cells.

8. A method for producing a cell-containing fine pattern sheet comprising cells and a support comprising a bioabsorbable material that has a cell adhesion protein-containing layer on the surface thereof, comprising the steps of:

a) providing endothelial cells;

b) contacting said cells with the surface of a substrate for cell arrangement having a cell adhesiveness-variation pattern comprising cell adhesiveness promoted regions having a water contact angle from 10° to 40°, and cell adhesiveness inhibited regions, wherein said cells adhere solely to the cell adhesiveness promoted regions of said substrate;

c) culturing said cells adhering to the surface of said substrate;

d) contacting said substrate adherent cells with a cell adhesion protein-containing layer on the surface of a support wherein said substrate adherent cells also adhere to said support, wherein the cell adhesion protein-containing layer has not been subjected to a patterning treatment;

e) culturing said substrate adherent cells between the substrate for cell arrangement and the cell-adhesion protein containing layer on the surface of said support to form a cell pattern comprising a lumen on said support,

f) removing the substrate for cell arrangement to transfer said substrate adherent cells to said support;

wherein the support comprises an amnion in which a layer composed of cells is removed, and the support is supported by a biodegradable polymer material, wherein the amnion consists of stratum compactum and basal lamina,

and wherein the cell-containing fine pattern sheet has a pattern not more than 3 mm in size and is an implant.

9. The method according to claim 8 , wherein the support further comprises a biodegradable polymer material and wherein the amnion is supported by the material.

10. The method according to claim 8 , wherein the cell adhesiveness-variation pattern is formed with a cell adhesiveness-variable layer comprising a cell adhesiveness-variable material that experiences a change in cell adhesiveness due to photocatalytic action upon energy irradiation.

11. The method according to claim 8 , wherein the cell adhesiveness-variable layer is a photocatalyst-containing cell adhesiveness-variable layer comprising a photocatalyst and a cell adhesiveness-variable material.

12. The method according to claim 8 , wherein the cell adhesiveness-variable layer has a photocatalyst treatment layer containing photocatalyst and a cell adhesiveness-variable material layer containing a cell adhesiveness-variable material, which is formed on the photocatalyst treatment layer.

13. The method according to claim 8 , wherein the cell adhesiveness-variation pattern is formed via energy irradiation after placing the cell adhesiveness-variable layer that contains a cell adhesiveness-variable material and a photocatalyst-containing layer that contains a photocatalyst in a manner such that both layers are opposed to each other.

14. The method according to claim 8 , wherein the cells with adhesiveness to a substrate for cell arrangement are vascular endothelial cells.