Hydrolysate of avian cartilage, process of preparation and uses thereof
This invention relates to a hydrolysate of avian cartilage comprising 45% to 70% by weight of hydrolysed type II collagen, 9% to 15% by weight of chondroitin sulphate, 0.5% to 2% in weight of hyaluronic acid; with a composition of amino acids in which valine represents 2.7% to 3.3%, isoleucine represents 2.0 to 2.4, phenylalanine represents 2.2% to 2.6%, lysine represents 3.8% to 4.2%, tryptophane represents 0.4% to 0,6%, hydroxyproline represents 5.5% to 8.7%, hydroxylysine represents 0.7% to 1.8%, and in which the molar ratio between hydroxyproline and hydroxylysine is between 5.0 and 8.0; and having an average molecular weight of the peptidic fraction between 500 and 1000 Daltons. The invention also relates to a process for preparing said hydrolysate, and its use as a food complement, and/or as a medicament, particularly for treatment or prevention of joint pain.
1. A hydrolysate of avian cartilage comprising a peptidic fraction and glycosaminoglycans, wherein:
a) it comprises:
45% to 70% by weight of hydrolysed type II collagen,
9% to 15% by weight of chondroitin sulphate,
0.5% to 2% by weight of hyaluronic acid;
b) said peptidic fraction comprises:
2.7% to 3.3% by weight of valine,
2.0% to 2.4% by weight of isoleucine,
2.2% to 2.6% by weight of phenylalanine,
3.8% to 4.2%, by weight of lysine
0.4% to 0.6% by weight of tryptophane,
5.5% to 8.7% by weight of hydroxyproline,
0.7% to 1 .8% by weight of hydroxylysine,
and the molar ratio of hydroxyproline to hydroxylysine is between 5.0 and 8.0;
c) the average molecular weight of said peptidic fraction is between 500 and 1000 Daltons.
2. The hydrolysate according to claim 1 , wherein it is prepared from a mix of 70%-100% by weight of bone and joint cartilage and 0%-30% by weight of sternum cartilage.
3. A process for preparing a hydrolysate of avian cartilage according to claim 1 , comprising:
a) supplying a raw material composed of 70%-100% by weight of bone and joint cartilage (including sternal) and 0%-30% by weight of sternum cartilage,
b) mixing this raw material with an aqueous solution with a pH between 5.5 and 7.5,
c) hydrolysing the mix of raw material in water with a proteolytic enzyme during 1 hour to 2 hours, preferably during 1 hour and 30 minutes to 2 hours, at a temperature between 65° C. and 75° C.,
d) inactivating the proteolytic enzyme for 10 to 20 minutes at least 85° C.,
e) filtering the reaction mix containing the hydrolysate,
f) concentrating the reaction mix,
g) performing final sterilization of the reaction mix at about 130° C. for at least about 30 seconds, and
h) drying the reaction mix to obtain the hydrolysate in powder form.
4. A food complement comprising the hydrolysate according to claim 1 .
5. A medicament comprising the hydrolysate according to claim 1 .
6. A method for treating joint pain, comprising administering to a patient an effective amount of the hydrolysate according to claim 1 .
7. The method according to claim 6 , wherein the origin of joint pain is degeneration of cartilage related to age, excessive strain of joints due to intensive practice of a sport, or excessive strain of joints due to obesity.