IP Library Granted Patent US 7,737,172
Granted Patent B2
US 7,737,172 · App. 11/463,421 · Granted Jun 15, 2010

3-3-di-substituted-oxindoles as inhibitors of translation initiation

Assignee: President and Fellows of Harvard College
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Quick Facts
Patent No.
US 7,737,172
App. No.
11/463,421
Granted
Jun 15, 2010
Kind
B2
Abstract

Compositions and methods for inhibiting translation using 3-(5-tert-Butyl-2-Hydroxy-phenyl)-3-phenyl-1,3-dihydro-indol-2-one and/or its derivatives are provided. Compositions, methods and kits for treating (1) cellular proliferative disorders, (2) non-proliferative, degenerative disorders, (3) viral infections, and/or (4) disorders associated with viral infections, using 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3-dihydro-indol-2-one and/or its derivatives are described.

Claims (116)

1. A method of treating cancer in a human or non-human mammal in need thereof, the method comprising administering to the human or non-human mammal a compound of formula III

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3 ′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph, and

wherein the cancer is a member selected from the group consisting of breast cancer, lung cancer, melanoma, prostate cancer, leukemia, multiple myeloma, plasmocytoma, pancreatic cancer and colon cancer.

2. The method of claim 1 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one.

3. The method of claim 1 , wherein the compound is administered by inhalation, transdermally, orally, rectally, transmucosally, intestinally, parenterally, intramuscularly, subcutaneously or intravenously.

4. A method of treating cancer in a human or non-human mammal in need thereof, the method comprising administering to the human or non-human mammal a compound of formula IV

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe, and

wherein the cancer is a member selected from the group consisting of breast cancer, lung cancer, melanoma, prostate cancer, leukemia, multiple myeloma, plasmocytoma, pancreatic cancer and colon cancer.

5. The method of claim 4 , wherein the compound is administered by inhalation, transdermally, orally, rectally, transmucosally, intestinally, parenterally, intramuscularly, subcutaneously or intravenously.

6. A method of treating cancer in a human or non-human mammal in need thereof, the method comprising administering to the human or non-human mammal a compound of formula VI

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H, and

wherein the cancer is a member selected from the group consisting of breast cancer, lung cancer, melanoma, prostate cancer, leukemia, multiple myeloma, plasmocytoma, pancreatic cancer and colon cancer.

7. The method of claim 6 , wherein the compound is administered by inhalation, transdermally, orally, rectally, transmucosally, intestinally, parenterally, intramuscularly, subcutaneously or intravenously.

8. A method of treating cancer in a human or non-human mammal in need thereof, the method comprising administering to the human or non-human mammal a compound of formula VIII

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph, and

wherein the cancer is a member selected from the group consisting of breast cancer, lung cancer, melanoma, prostate cancer, leukemia, multiple myeloma, plasmocytoma, pancreatic cancer and colon cancer.

9. The method of claim 8 , wherein the compound is administered by inhalation, transdermally, orally, rectally, transmucosally, intestinally, parenterally, intramuscularly, subcutaneously or intravenously.

10. The method of claim 8 wherein the compound is 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

11. A method of treating breast cancer in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

R 2 is selected from the group consisting of 2-OH, 4-OH, 4-t-Bu and H with the proviso that R 1 and R 2 cannot both be H or 4-OH; or

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

12. A method of treating lung cancer in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

R 2 is selected from the group consisting of 2-OH, 4-OH, 4-t-Bu and H with the proviso that R 1 and R 2 cannot both be H or 4- 0 H; or

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

13. A method of treating melanoma in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3 40 -n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3 40 -n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

14. A method of prostate cancer in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H; or

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

15. A method of treating leukemia in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

16. A method of treating multiple myeloma in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

17. A method of treating plasmocytoma in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

18. A method of treating pancreatic cancer in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3′-O(CH 2 ) 4 N 3 ,

3′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

19. A method of treating colon cancer in a human or non-human mammal, the method comprising administering to the human or non-human mammal a compound of

wherein X is selected from the group consisting of oxygen, NH and CH 2 ; and

R is selected from the group consisting of 3′-CH 2 CH(CO 2 Me)NH 2 , 3′-CH 2 CH(CO 2 Me)NHFmoc, 3′-t-Bu, 3′-OH, 3′-O(CH 2 ) 3 CHCH 2 , 3′-O(CH 2 ) 4 Br, 3′-O(CH 2 ) 4 NMe 2 , 3-O(CH 2 ) 4 N 3 ,

3-′-O(CH 2 ) 4 NH 2 , 3′-CF 3 , 3′-CH 3 , 3′-n-heptyl, 3′-n-nonyl, 3′-i-Bu, 3′-i-propyl, 3′-n-propyl, 3′-n-octyl, 3′-Et, 3′-n-pentyl, 3′-n-Bu, 3′-n-hexyl, 3′-n-cyclopentyl, 3′-n-cyclohexyl, 4-NHSO 2 (4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph;

wherein R is selected from the group consisting of 4-F, 4-O(CH 2 ) 3 CHCH 2 , 3-Cl, 3-F, 3-Me, 2-Me and 2-OMe;

wherein R 1 is selected from the group consisting of 7-Br, 4-Cl, 5-Br, 5-NO 2 , 5-I, 5-OMe, 5-Cl, 7-CF 3 , 5-NH 2 , 4-Br, 6-Br, H, 7-OMe, 7-I, 5-Et, 7-Et, 4-Et, 6-Et, 5-F, 5-N 3 , 7-COOH, 7-CO 2 H, 4-Cl, 6-Cl and 5-NHSO 2 -1-napthyl; and R 2 is H or 7-CO 2 H;

wherein R 1 is selected from the group consisting of H and 7-CO 2 H;

R 2 is selected from the group consisting of H, 2-Me, 3′-Me, 3-F and 3-CF 3 ; and

R 3 is selected from the group consisting of H, 2-NHFmoc, 2-NHSO 2 CH 2 Ph, 2-NHSO 2 CH 3 , 2-NHSO 2 (4-tBu)Ph, 2-NHSO 2 (4-NHAc)Ph, 2-NHSO 2 (3-CF 3 )Ph, 2-NHSO 2 (4-NO 2 )Ph, 2-NHSO 2 (4-OMe)Ph, 2-NHSO 2 (4-Br)Ph, 2-NHSO 2 (4-I)Ph, 2-NHSO 2 (4-Ph)Ph, 2-NHSO 2 (4-OPh)Ph, 2-NHAc, 2-NHCO(4-tBu)Ph and 3′-NHSO 2 (4-tBu)Ph.

20. The method of claim 11 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

21. The method of claim 12 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

22. The method of claim 13 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

23. The method of claim 14 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

24. The method of claim 15 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

25. The method of claim 16 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

26. The method of claim 17 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

27. The method of claim 18 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

28. The method of claim 19 wherein the compound is 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide.

29. A method of treating cancer in a human or non-human mammal in need thereof, the method comprising administering to the human or non-human mammal 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one or 4-tert-butyl-N-(5-hydroxy-4-methyl-2-(2-oxo-3-phenylindolin-3-yl)phenyl)benzenesulfonamide wherein the cancer is a member selected from the group consisting of breast cancer, lung cancer, melanoma, prostate cancer, leukemia, multiple myeloma, plasmocytoma, pancreatic cancer and colon cancer.

30. The method of claim 29 comprising administering 3-(5-tert-butyl-2-hydroxy-phenyl)-3-phenyl-1,3,-dihydro-indol-2-one.

Assignments (2)
CONFIRMATORY LICENSE Recorded Jul 30, 2018
From: HARVARD UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 046646/0899 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 16, 2007
From: HALPERIN, JOSE A.; NATARAJAN, AMARNATH; AKTAS, HUSEYIN; FAN, YUN-HUA; CHEN, HAN
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 018761/0177 →
Continuity (3)
Continuation PCTUS200500437300 · Feb 11, 2005
Provisional Application 6054438400 · Feb 13, 2004
Related Publication 20070099976A1 · May 3, 2007