Purified component of blue-green algae and method of use
Disclosed herein are extracts of blue green algae, such as Aphanizomenon flos aquae , that are enriched for a selectin ligand, such as an L-selectin ligand. Selectin ligands isolated from blue-green algae cells are disclosed herein. Methods are described for isolating these selectin ligands. The purified selectin ligands are of use in inducing stem cell mobilization in a subject. Thus, methods for inducing stem cell isolation that include administering a therapeutically effective amount of the extract enriched form the selectin ligand, or an isolated selectin ligand, are disclosed herein.
1. A composition comprising a first and a second component of Aphanizomenon flos aquae , wherein the first component consists of a dried form of a solvent extract of fresh, dehydrated, or preserved Aphanizomenon flos aquae , wherein the extract comprises a L-selectin ligand that binds specifically to L-selectin and inhibits fucoidan-mediated expression of the CXCR4 receptor, and where the solvent consists of water or buffered saline; and
wherein the second component consists of a dried form of a second solvent extract of fresh, dehydrated, or preserved Aphanizomenon flos aquae , wherein the second solvent comprises ethanol.
2. A composition comprising a therapeutically effective amount of the composition of claim 1 in a pharmacologically acceptable carrier.
3. The composition of claim 2 , wherein the second solvent extract is produced by suspending dried Aphanizomenon flos aquae in about 10 to about 20 percent ethanol at about 50° C. to about 60° C.
4. The composition of claim 2 , comprising 1 gram to about 2 grams of the first component of Aphanizomenon flos aquae , and about 100 milligrams to about 500 milligrams of the second component of Aphanizomenon flos aquae.
5. The composition of claim 4 , comprising about 1 gram of the first component of Aphanizomenon flos aquae , and about 150 milligrams of the second component of Aphanizomenon flos aquae.
6. The composition of claim 5 , produced by the process of
contacting a first amount of Aphanizomenon flos aquae in a solvent consisting of water or phosphate buffered saline for about half an hour to about 12 hours to produce a first extract;
removing solid material from the first extract;
drying the first extract to produce a solid composition comprising a L-selectin ligand;
incubating a second amount of Aphanizomenon flos aquae in 10 percent ethanol at about 50° C. for about one hour to produce a second extract;
removing solid material from the second extract;
drying the second extract to produce a solid form of the second extract; and
mixing a therapeutically effective amount of the solid composition comprising the L-selectin ligand and a therapeutically effective amount of the solid form of the second extract.
7. A composition comprising about 1 gram of a first component of Aphanizomenon flos aquae and about 150 milligrams of a second component of Aphanizomenon flos aquae , wherein the first component consists of a dried form of a first solvent extract of fresh, dehydrated, or preserved Aphanizomenon flos aquae , wherein the first solvent extract comprises a L-selectin ligand that binds specifically to L-selectin and inhibits fucoidan-mediated expression of the CXCR4 receptor, and where the first solvent consists of water or buffered saline; and wherein the second component consists of a dried form of a second solvent extract of fresh, dehydrated, or preserved Aphanizomenon flos aquae , wherein the second solvent comprises about 10 percent to about 20 percent ethanol.
8. The composition of claim 7 , where the first solvent consists of water.
9. The composition of claim 7 , where the first solvent consists of buffered saline.
10. The composition of claim 7 , wherein the second solvent comprises about 10 percent ethanol.
11. The composition of claim 7 , wherein the second solvent comprises about 20 percent ethanol.
12. The composition of claim 7 , wherein the composition is a solid.
13. The composition of claim 10 wherein the solid is encapsulated.
14. A liquid composition comprising 1 gram of the composition of claim 7 dissolved in water.