Modified zinc finger binding proteins
Disclosed herein are compositions and method comprising non-canonical (e.g., non-C2H2) zinc finger proteins.
1. A non-naturally-occurring zinc-finger binding protein comprising a non-canonical zinc finger, wherein:
said non-canonical zinc finger component comprises the sequence X 3 -Cys-X 2-4 -Cys-X 12 -His-X 1-7 -Z-X 4 (SEQ ID NO:121), where X is any amino acid, where Z is C, where residues 6 to 12 of X 12 are engineered to bind to a target nucleic acid sequence, wherein X 1-7 is selected from the group consisting of SETG (residues 2-5 of SEQ ID NO:12), LKSLTP (residues 2-7 of SEQ ID NO:13), SEN, VRI, KRS, KRL, LRW, ITT, IKG and IGG.
2. The non-naturally-occurring zinc-finger binding protein of claim 1 , wherein the target sequence is a promoter sequence.
3. The non-naturally-occurring zinc-finger binding protein of claim 1 , wherein the zinc finger binding protein comprises three zinc fingers.
4. The non-naturally-occurring zinc-finger binding protein of claim 3 , wherein the third zinc finger comprises a non-canonical zinc finger.
5. The non-naturally-occurring zinc-finger binding protein of claim 1 , further comprising a functional domain.
6. The non-naturally-occurring zinc-finger binding protein of claim 5 , wherein the functional domain is an endonuclease.
7. A plant cell comprising the non-naturally-occurring zinc-finger binding protein of claim 1 .
8. The non-naturally occurring zinc finger binding proteins of claim 5 , wherein the functional domain is a repressive domain.
9. The non-naturally occurring zinc finger binding proteins of claim 5 , wherein the functional domain is an activation domain.