Flow cytometry analysis across optical fiber
A flow cytometer type apparatus and method for analyzing a fluid, where the fluid is fed through a passageway within an optical fiber and the light is guided by the fiber across the passageway and intersects the fluid therein. The apparatus includes an optical fiber with a passageway traversing it, a channeling system to channel the fluid medium within the passageway, a light source to propagate light within the optical fiber and across the passageway, and a detection system for detecting an intensity of the light exiting the fiber. The method includes channeling the fluid medium through the passageway, propagating light within the optical fiber, and detecting an intensity of the light output. Preferably, the fluid includes small bodies, like bacteria which are analyzed by assessing the detected exiting light intensity.
1. An apparatus for analyzing a fluid, the apparatus comprising:
an optical fiber with a core extending along a longitudinal optical axis of the optical fiber for a propagation of light along the optical axis, said core being solid except for a transversal passageway traversing the optical fiber directly through the optical axis including through said core, the passageway being a hole traversing the optical fiber in a substantially orthogonal direction relative to said optical axis of the optical fiber and having at least a portion with a taper shape;
a channeling system adapted to channel the fluid through the passageway;
a light source system optically coupled to the optical fiber to propagate light in the solid core of the optical fiber, and across the passageway; and
a light detection system optically coupled to the optical fiber for detecting an intensity of the light exiting the fiber after having propagated across the fluid in the passageway.
2. The apparatus of claim 1 , wherein said fluid contains small bodies.
3. The apparatus of claim 2 , wherein the small bodies are biological specimens.
4. The apparatus of claim 3 , wherein the small bodies are bacteria.
5. The apparatus of claim 1 , wherein said fluid is a homogeneous solution including fluorophores.
6. The apparatus of claim 1 , wherein the optical fiber is a multimode optical fiber having a core diameter of 62.5 μm, and the hole has a diameter between 10 μm and 50 μm.
7. The apparatus of claim 2 , wherein the light detection system further comprises a presence detector for detecting a presence of a small body in the fluid in the hole based on an impulse of the detected exiting light intensity.
8. The apparatus of claim 2 , wherein the light detection system further comprises a dimension detector for determining at least one of a size and a length of the small body based respectively on at least one of an amplitude and a duration of an impulse in the detected exiting light intensity.
9. The apparatus of claim 2 , wherein the light detection system further comprises a status detector for detecting a fluorescence intensity emitted by a biological specimen in the hole, whereby a vitality status of the biological specimen is determined by an impulse in the fluorescence intensity detected.
10. The apparatus of claim 1 wherein the light source system comprises a UV source and the fluid includes fluorophores.
11. The apparatus of claim 1 wherein the light detection system is adapted to detect light intensity at three different wavelengths.
12. The apparatus of claim 1 wherein the light detection system has a collimation lens receiving light exiting the optical fiber, a diffraction grating receiving the collimated light and diffracting it, and at least one focusing lens and photodetector assembly disposed at a predetermined angle to said diffraction grating for detecting an intensity of light of a predetermined wavelength diffracted by the diffraction grating.
13. The apparatus of claim 1 wherein the light detection system has an optical fiber spectrograph, a collimation lens for receiving light exiting the optical fiber, a filter for filtering the wavelengths of exiting light corresponding to the light source system, an injection lens for injecting the filtered light into the optical fiber spectrograph.
14. The apparatus of claim 1 wherein the channeling system comprises a container of the fluid and a container aperture at least partly coaxial with the passageway, and an intermediate plate between the optical fiber and the container having an intermediate aperture of a size intermediate between that of the passageway and that of the container aperture, the intermediate aperture being disposed along the direction of the passageway, whereby the fluid is successively funneled into the passageway through the container and intermediate apertures.
15. The apparatus of claim 14 wherein the channeling system further comprises a pressure controller for controlling a pressure in the container.
16. The apparatus of claim 1 wherein the optical fiber is a double clad optical fiber.
17. The apparatus of claim 1 wherein the optical fiber further comprises a second core.
18. A method for analyzing a fluid, the method comprising:
channeling the fluid through a transversal passageway traversing through a core extending along a longitudinal optical axis of an optical fiber and which is solid except for the passageway, the passageway being a hole traversing the optical fiber in a substantially orthogonal direction relative to said optical axis of the optical fiber and having at least a portion with a taper shape, said optical axis being an axis for a propagation of light in said optical fiber;
propagating light into the core of the optical fiber and across the passageway and generating a light output; and
detecting an intensity of the light output.
19. The method as claimed in claim 18 , wherein small bodies are provided in said fluid, further comprising determining a presence of a small body in the passageway by detecting an impulse in the detected output light intensity.
20. The method as claimed in claim 18 , wherein small bodies are provided in said fluid, further comprising determining a dimension of a small body in the passageway by deducing the size and length of the small body from the amplitude and duration, respectively, of an impulse in the detected output light intensity.
21. The method as claimed in claim 18 , wherein small bodies are provided in said fluid, further comprising determining a vitality state of a small body in the hole as being one of alive and dead by detecting an intensity of a spectral portion of the light output generated by a fluorescence emitted by the small body.
22. The method as claimed in claim 18 wherein the optical fiber has a second core and the light is propagated in both cores thus generating two light outputs, further comprising analyzing the light intensity emanating from the light outputs using an interferogram.
23. A flow cytometer for analyzing a fluid by channeling the fluid and intersecting the channeled fluid with light for interaction therewith and to be detected thereafter, characterized in that: the fluid is channeled through a transversal hole defined substantially orthogonally relative to a longitudinal optical axis of an optical fiber, said optical fiber has a core which is solid except for said hole and said hole has at least a portion with a taper shape, said optical axis being an axis for the propagation of light in said optical fiber, and the light is guided longitudinally within the optical fiber along said optical axis and intersects the channeled fluid.