Methods for screening for compounds that modulate PD-1 signaling
View Patent ↗Disclosed are screening assays for identifying compounds which modulate the activity of, or signaling via, B7-4, or PD-1, especially which modulate the binding of B7-4 or PD-1 to a target molecule.
1. A method for screening for compounds which modulate PD-1 signaling comprising:
a) contacting a T cell activated in the presence of PD-L1 with a test compound;
b) contacting a T cell activated in the absence of PD-L1 with the test compound; and
c) determining the ability of the test compound to modulate phosphorylation of SHP-2;
wherein modulation of phosphorylation of SHP-2 in the presence of PD-L1 and not in the absence of PD-L1 indicates modulation of PD-1 signaling, thereby identifying a compound which modulates PD-1 signaling.
2. The method of claim 1 wherein the test compound upmodulates PD-1 signaling and thereby upmodulates SHP-2 phosphorylation.
3. The method of claim 1 wherein the test compound downmodulates PD-1 signaling and thereby downmodulates SHP-2 phosphorylation.