Membrane bioreactor
View Patent ↗The invention provides a membrane comprising a gel reinforced by a support. The membrane has opposing surfaces and a thickness between said surfaces. The gel communicates between the opposing surfaces and allows diffusion of a nutrient solution through the membrane. A bioreactor is also provided, comprising a membrane-supporting structure, and a membrane according to the invention supported on the membrane-supporting structure.
1. A bioreactor comprising:
a membrane-supporting structure; and
a membrane supported on the membrane-supporting structure, said membrane having a nutrient face, a gas face and a thickness between said faces, said membrane having an immobilised biolayer in a location selected from on the gas face and in the membrane near the gas face,
wherein the membrane comprises a gel reinforced by a support between the gas face and the nutrient face, and the gel providing communication between the nutrient face and the gas face of the membrane allowing diffusion of a nutrient solution through the membrane and the membrane allows diffusion of a nutrient solution from the nutrient face to the immobilised biolayer,
and wherein the membrane, when supported by the supporting structure, has one configuration selected from the group consisting of:
a pair of membranes disposed so as to define an inside region between the membranes of the pair;
the membrane disposed adjacent to a non-porous support disposed so as to define an inside region between the membrane and the non-porous support, said non-porous support comprising a material that is impervious to the nutrient solution; and
the membrane supported in a configuration in which a portion of the membrane is parallel to another portion of the membrane so as to define an inside region between the two portions,
and wherein the nutrient face abuts the inside region.
2. The bioreactor of claim 1 wherein the inside region between the membranes of the pair abuts the nutrient face of each of the membranes of the pair.
3. The bioreactor of claim 1 , wherein the membrane is supported on the membrane-supporting structure in an orientation selected from the group consisting of vertically, non-horizontally, at a non-zero angle to the horizontal, at an angle to the horizontal between about 30° and 90° , at an angle to the horizontal between about 45° and 90° , at an angle to the horizontal between about 60° and 90° at an angle to the horizontal between about 45° and 60° , at an angle to the horizontal of about 30° , at an angle to the horizontal of about 45° , and at an angle to the horizontal of about 60°.
4. The bioreactor of claim 1 wherein the membrane is supported vertically.
5. The bioreactor of claim 1 wherein the membrane is a planar membrane, and wherein the membranes of the pair are supported in a configuration such that a portion of one membrane of the pair is parallel to a portion of the other membrane of the pair.
6. The bioreactor of claim 1 comprising one or more spacers for maintaining a distance between the membranes of the pair.
7. The bioreactor of claim 1 having an inlet for admitting the nutrient solution to the nutrient face of the membrane, an outlet for removing the nutrient solution from the nutrient face of the membrane, and a recycling system for recycling the nutrient solution from the outlet to the inlet.
8. The bioreactor of claim 7 wherein the recycling system is capable of preventing access of oxygen to the liquid.
9. The bioreactor of claim 1 also comprising an oxygen remover for removing oxygen from the nutrient solution.
10. The bioreactor of claim 1 having means for removing solid matter from the membrane.
11. The bioreactor of claim 1 wherein the biolayer is both on the gas face and in the membrane near the gas face.
12. The bioreactor of claim 1 wherein the biolayer is exposed such that cells from the immobilised biolayer may be removed once formed.
13. A method for operating a bioreactor that comprises:
a membrane-supporting structure; and
a membrane supported on the membrane-supporting structure, said membrane having a nutrient face, a gas face and a thickness between said faces, said membrane having an immobilised biolayer in a location selected from on the gas face and in the membrane near the gas face;
wherein the membrane comprises a gel reinforced by a support between the gas face and the nutrient face, and the gel providing communication between the nutrient face and the gas face of the membrane allowing diffusion of a nutrient solution through the membrane and the membrane allows diffusion of a nutrient solution from the nutrient face to the immobilised biolayer, and
wherein the membrane when supported by the supporting structure has one configuration selected from the group consisting of:
a pair of membranes disposed so as to define an inside region between the membranes of the pair;
the membrane disposed adjacent to a non-porous support disposed so as to define an inside region between the membrane and the non-porous support, said non-porous support comprising a material that is impervious to the nutrient solution; and
the membrane supported in a configuration in which a portion of the membrane is parallel to another portion of the membrane so as to define an inside region between the two portions, and
wherein the nutrient face abuts the inside region, said method comprising:
exposing the nutrient face of the membrane to a nutrient solution,
exposing the biolayer to a gas, and
allowing the nutrient solution to diffuse from the nutrient face of the membrane to the biolayer.
14. The method of claim 13 wherein, in use, the nutrient solution is substantially anoxic.
15. The method of claim 13 wherein the nutrient solution is substantially anoxic.
16. The method of claim 13 wherein the step of allowing the nutrient solution to diffuse is performed with a pressure across the membrane of less than 0.05 atmosphere.
17. The method of claim 13 wherein the gas contains oxygen.
18. The method of claim 13 wherein the step of exposing the nutrient face of the membrane to a nutrient solution comprises recycling the nutrient solution past the nutrient face.
19. The method of claim 13 wherein the nutrient solution does not pass through the membrane into a gas region adjoining the gas face of the membrane.
20. The method of claim 13 wherein the biolayer and the composition of the nutrient solution are chosen for a purpose selected from the group consisting of producing pharmaceuticals, producing antibodies, producing vaccine components, producing food material, producing cells, producing enzymes and for the purpose of removing, degrading or converting components in the nutrient solution.
21. The method of claim 13 wherein the biolayer and the composition of the nutrient solution are chosen for production of an antibiotic.
22. The method of claim 13 also comprising the step of removing oxygen from the nutrient solution.
23. The method of claim 13 additionally comprising the step of isolating a product of the bioreactor.
24. The method of claim 23 wherein the step of isolating comprises separating the product from the nutrient solution.
25. The method of claim 23 wherein the step of isolating comprises harvesting solid matter from the gas face.
26. The method of claim 13 additionally comprising:
introducing a second liquid to the nutrient face of the membrane,
exposing the membrane to the second liquid for a second period of time, and
separating a product from the second liquid.
27. The method of claim 13 wherein the biolayer includes an outer surface in direct contact with the gas.
28. The method of claim 13 wherein the pressure of the gas at the gas face is between about 0.9 and 1.1 atmospheres.