Means and methods for controlling flowering in plants
Described are means and methods for controlling flowering in plants. In particular, described are nucleic acid molecules which, when expressed in sense orientation or in antisense orientation, respectively, in plants lead to a prevention of flowering. Moreover, a method for controlling flowering in plants is provided which comprises the inducible restoration of flowering in plants in which flowering is prevented.
1. An isolated polynucleotide which, when expressed in sense orientation in plants leads to a prevention of flowering, selected from the group consisting of
(a) polynucleotides comprising a nucleotide sequence encoding a polypeptide with the amino acid sequence of SEQ ID NO:2;
(b) polynucleotides comprising the coding region of the nucleotide sequence shown in SEQ ID NO:1; and
(c) polynucleotides comprising a nucleotide sequence having a sequence identity of at least 95% to the coding region of SEQ ID NO: 1 and polynucleotides comprising a nucleotide sequence encoding a polypeptide having a sequence identity of at least 95% to the entire amino acid sequence of SEQ ID NO: 2, wherein said nucleotide sequence when expressed in sense orientation in plants leads to the production of the encoded polypeptide and to a prevention of flowering in comparison to wild-type plants.
2. The isolated polynucleotide of claim 1 which is DNA or RNA.
3. A recombinant nucleic acid molecule comprising the polynucleotide of claim 1 .
4. The recombinant nucleic acid molecule of claim 3 further comprising expression control sequences operably linked to said polynucleotide.
5. A vector comprising the polynucleotide of claim 1 or the recombinant nucleic acid molecule of claim 4 .
6. The vector of claim 5 further comprising expression control sequences operably linked to said polynucleotide.
7. A method for producing genetically engineered host cells comprising introducing the isolated polynucleotide of claim 1 , or the recombinant nucleic acid molecule of claim 4 into isolated host cells.
8. An isolated host cell which is genetically engineered with the polynucleotide of claim 1 .
9. The host cell of claim 8 which is a bacterial, yeast, fungus, plant or animal cell.
10. A method for producing a transgenic plant comprising the steps of
(a) introducing the polynucleotide of claim 1 into the genome of a plant cell; and
(b) regenerating the cell of (a) to a transgenic plant.
11. A transgenic plant, or plant tissue comprising the plant cell of claim 9 or obtainable by the method of claim 10 .
12. The transgenic plant of claim 11 which, upon an increased amount of the protein encoded by said polynucleotide as compared to a corresponding wild-type plant, shows a prevention of flowering.
13. Propagation material or harvestable parts of the transgenic plant of claim 11 comprising said plant cell.
14. The propagation material of claim 13 which is a seed.
15. The isolated polynucleotide of claim 1 , which is selected from the group consisting of polynucleotides comprising a nucleotide sequence having a sequence identity of at least 97% to the coding region of SEQ ID NO: 1 and polynucleotides comprising a nucleotide sequence encoding a polypeptide having a sequence identity of at least 97% to the entire amino acid sequence of SEQ ID NO: 2, wherein said nucleotide sequence when expressed in sense orientation in plants leads to the production of the encoded polypeptide and to a prevention of flowering in comparison to wild-type plants.
16. The isolated polynucleotide of claim 1 , which is selected from the group consisting of polynucleotides comprising a nucleotide sequence having a sequence identity of at least 99% to the coding region of SEQ ID NO: 1 and polynucleotides comprising a nucleotide sequence encoding a polypeptide having a sequence identity of at least 99% to the entire amino acid sequence of SEQ ID NO: 2, wherein said nucleotide sequence when expressed in sense orientation in plants leads to the production of the encoded polypeptide and to a prevention of flowering in comparison to wild-type plants.