Reagent containing protease reaction promoter and/or colorant stabilizer
Stabilizers for colorants used for detection of enzymatic reactions, having a cyclodextrin and a catalase; reagents including stabilizers; and methods of use of such stabilizers and reagents.
1. A method for stabilizing a kit of compositions comprising a first composition and a second composition used for the measurement of glycated hemoglobin or glycated albumin in a sample comprising:
making a protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and a cyclodextrin coexist together in the absence of a sample containing glycated hemoglobin or glycated albumin, and thereby providing the first composition comprising the protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and the cyclodextrin with no sample containing glycated hemoglobin or glycated albumin, and
providing the second composition containing an oxidase which oxidizes a glycated amino acid and/or a glycated peptide,
wherein the first and second compositions of the kit remain stable in a liquid state for 6 months or longer under refrigeration in the absence of a sample containing glycated hemoglobin or glycated albumin.
2. The method according to claim 1 , wherein the cyclodextrin is a β-cyclodextrin.
3. The method according to claim 1 , wherein the first composition containing the protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and the cyclodextrin further comprises a buffer in a concentration of 100 mM or less.
4. The method according to claim 3 , wherein the first composition containing the protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and the cyclodextrin further comprises a buffer in a concentration of 80 mM or less.
5. A method of measuring glycated hemoglobin or glycated albumin in a blood sample, comprising:
adding to the blood sample,
the first composition stabilized by the method according to claim 1 , thus providing a mixture of the blood sample and the first composition,
adding to the mixture the second composition according to claim 1 , and
detecting through the activity of the oxidase which oxidizes a glycated amino acid and/or a glycated peptide, the presence of glycated amino acids and/or glycated peptides cleaved from glycated hemoglobin or glycated albumin present in the blood sample.
6. The method according to claim 5 , wherein the cyclodextrin is a β-cyclodextrin.
7. The method according to claim 5 , wherein the first composition containing the protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and the cyclodextrin further comprises a buffer in a concentration of 100 mM or less.
8. The method according to claim 7 , wherein the first composition containing the protease, 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine, and the cyclodextrin further comprises a buffer in a concentration of 80 mM or less.