Root-specific and xylem parenchyma-specific promoter
View Patent ↗The invention relates to a promoter that is provided with tissue-specific activity and is more active in xylem parenchyma cells of plant roots than it is in other cells of said plant. The inventive promoter allows transgenic plants to be produced with particular characteristics: (a) improved xylem charging and discharging processes in the root; (b) improved nitrogen supply; (c) reduced accumulation of harmful nitrogen in the root; (d) improved resistance to salt; (e) improved resistance to stress due to dry conditions; (f) improved tolerance to frost; (g) modified Na+/K+ concentration in the root; (h) greater resistance/tolerance to pathogens.
1. A promoter, comprising a nucleotide sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) a nucleotide sequence complementary to the full length of the nucleotide sequence according to SEQ ID NO: 1;
(c) a nucleotide sequence having transcriptional promoter activity, which hybridizes under stringent hybridizing conditions with the nucleotide sequence according to SEQ ID NO: 1; and
(d) a nucleotide sequence which hybridizes under stringent hybridizing conditions with the nucleotide sequence complementary to the nucleotide sequence according to SEQ ID NO: 1, wherein said sequence that hybridizes has transcriptional promoter activity, and wherein the stringent hybridizing conditions are either:
(1) hybridizing in 4×SSC at 65° C. and subsequent multiple washing in 0.1×SSC at 65° C. for a total of approximately 1 hour; or
(2) hybridizing at 68° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2×SSC and 0.1% SDS at 68° C.
2. A vector or mobile genetic element, containing the promoter according to claim 1 or a derivative thereof.
3. Monocot or dicot root cells, containing the promoter according to claim 1 or a derivative thereof.
4. A transgenic plant or part thereof, containing the promoter according to claim 2 or a derivative thereof.
5. Transgenic plant according to claim 4 , wherein the plant is Beta vulgaris.
6. A plant part according to claim 4 , wherein said plant part is a seed.
7. A method for producing a transgenic plant with one or more of the following characteristics:
a. improved loading/charging and unloading/discharging processes of the xylem in the root,
b. improved nitrogen supply,
c. reduced accumulation of “detrimental nitrogen” in the root,
d. improved salt resistance,
e. improved drought resistance,
f. improved frost tolerance,
g. modified Na+/K+ concentration in the root,
h. elevated resistance/tolerance to pathogens, said method comprising transforming a plant with a promoter according to claim 2 .