Nucleic acid sequences encoding luciferase for expression in filamentous fungi
View Patent ↗The present invention relates to genetic reporters. Specifically, the present invention is directed to a modified gene encoding a luciferase for high level expression in an organism with a bias for cytosine (C) or guanine (G) in the third position of the codon.
1. An isolated nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 1 and encoding a Photinus luciferase of SEQ ID NO:3 for expression in an organism with a bias for cytosine (C) or guanine (G) in the third position of the codon, wherein the nucleic acid sequence has a G and C content of at least 50% and an intron comprising SEQ ID NO:5.
2. A vector comprising the nucleic acid molecule of claim 1 .
3. An isolated host cell containing the vector of claim 2 .
4. A method for enhancing the level of a luciferase enzyme in an organism with a bias for C or G in the third position of the codon comprising introducing into an organism with a bias for C or G in the third position of the codon a nucleic acid molecule of claim 1 , thereby enhancing the level of luciferase enzyme produced in the organism.
5. A kit for expressing a luciferase enzyme in an organism with a bias for C or G in the third position of the codon comprising the nucleic acid molecule of claim 1 .
6. An isolated nucleic acid molecule comprising SEQ ID NO:1.
7. The nucleic acid molecule of claim 6 , further comprising an intron of SEQ ID NO:5.