Cell display of antibody libraries
The present invention relates to a viral vector encoding for a library of antibodies or antibody fragments that are displayed on the cell membrane when expressed in a cell. The present invention provides cells comprising the viral vector nucleic acids and methods of screening the libraries for antibodies or antibody fragments with desired characteristics.
1. A method for producing a library of cells displaying antibodies or fragments thereof on the cell surface comprising:
a. introducing into a population of cells a library of vectors encoding recombinant antibodies or fragments thereof that are displayed on the extracellular surface of the cell membrane; and
b. culturing the population of cells under conditions that allow expression of the antibodies or fragments thereof on the cell surface,
wherein the population of cells is a population of mammalian cells and wherein said antibodies or fragments thereof comprise the amino acid sequence of SEQ ID NO: 60.
2. The method of claim 1 , wherein the amino acid sequence of SEO ID NO: 60 is fused to the C-terminal end of the heavy chain or the light chain of the antibody.
3. The method of claim 1 , wherein said antibodies or fragments thereof are of an immunoglobulin type selected from the group consisting of IgA, IgE, IgM, IgD, IgY and IgG.
4. The method of claim 1 , wherein said antibodies or fragments thereof are murine antibodies, chimeric antibodies, humanized antibodies or human antibodies.
5. The method of claim 1 , wherein said antibodies or fragments thereof comprise an Fc region, wherein the Fc region is a naturally occurring Fc region or a variant Fc region comprising at least one amino acid substitution, insertion, deletion or combination thereof.
6. The method of claim 1 , wherein said antibodies or fragments thereof comprise a heavy chain variable region, a light chain variable region or both a heavy chain and a light chain variable region.
7. The method of claim 1 , wherein the mammalian cells are selected from the group consisting of NS0 cells, CHO cells, Vero cells, COS7 cells, and 293 cells.
8. The method of claim 1 , wherein said vectors are viral vectors.
9. The method of claim 8 , wherein said vectors are adenoviral vectors, adeno associated viral vectors, herpes viral vectors or lentiviral vectors.
10. A method of displaying a library of antibodies or fragments thereof on the cell surface comprising:
a. introducing into a population of cells a library of vectors encoding recombinant antibodies or fragments thereof wherein said antibodies or fragments thereof comprise the amino acid sequence of SEO ID NO: 60; and
b. culturing the population of cells under conditions that allow expression of the antibodies or fragments thereof on the cell surface,
wherein the population of cells is a population of mammalian cells.
11. The method of claim 10 , wherein the mammalian cells are selected from the group consisting of NS0 cells, CHO cells, Vero cells, COS7 cells, and 293 cells.
12. The method of claim 10 , wherein said antibody or fragment thereof is of an immunoglobulin type selected from the group consisting of IgA, IgE, IgM, IgD, IgY and IgG.
13. The method of claim 10 , wherein said antibody or fragment thereof is a murine antibody, chimeric antibody, humanized antibody or human antibody.
14. The method of claim 10 , wherein said antibody or fragment thereof comprises an Fc region, wherein the Fc region is a naturally occurring Fc region or a variant Fc region comprising at least one amino acid substitution, insertion, deletion or combination thereof.
15. The method of claim 10 , wherein the amino acid sequence of SEQ ID NO: 60 is fused to the C-terminal end of the heavy chain or the light chain of the antibody.